Clinics in Shoulder and Elbow

Korean Shoulder and Elbow Society (대한견·주관절의학회)
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Increased interleukin-6 and TP53 levels in rotator cuff tendon repair patients with hypercholesterolemia

  • Jong Pil Yoon (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Seung Gi Min (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Jin-Hyun Choi (Department of Bio-fibers and Materials Science, Kyungpook National University) ;
  • Hyun Joo Lee (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Kyeong Hyeon Park (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Sung Hyuk Yoon (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Seong Soo Kim (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University) ;
  • Seok Won Chung (Department of Orthopaedic Surgery, Konkuk University School of Medicine) ;
  • Hun-Min Kim (Korea Dyeing and Finishing Technology Institute (DYETEC)) ;
  • Dong Hyun Kim (Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University)
  • Received : 2022.04.22
  • Accepted : 2022.07.22
  • Published : 2022.12.01
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Abstract

Background: A previous study reported that hyperlipidemia increases the incidence of tears in the rotator cuff tendon and affects healing after repair. The aim of our study was to compare the gene and protein expression of torn rotator cuff tendons in patients both with and without hypercholesterolemia. Methods: Thirty patients who provided rotator cuff tendon samples were classified into either a non-hypercholesterolemia group (n=19, serum total cholesterol [TC] <200 mg/dL) and hypercholesterolemia group (n=11, serum TC ≥240 mg/dL) based on their concentrations of serum TC. The expression of various genes of interest, including COL1A1, IGF1, IL-6, MMP2, MMP3, MMP9, MMP13, TNMD, and TP53, was analyzed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, Western blot analysis was performed on the proteins encoded by interleukin (IL)-6 and TP53 that showed significantly different expression levels in real-time qRT-PCR. Results: Except for IGF1, the gene expression levels of IL-6, MMP2, MMP9, and TP53 were significantly higher in the hypercholesterolemic group than in the non-hypercholesterolemia group. Western blot analysis confirmed significantly higher protein levels of IL-6 and TP53 in the hypercholesterolemic group (p<0.05). Conclusions: We observed an increase in inflammatory cytokine and matrix metalloproteinase (MMP) levels in hypercholesterolemic patients with rotator cuff tears. Increased levels of IL-6 and TP53 were observed at both the mRNA and protein levels. We suggest that the overexpression of IL-6 and TP53 may be a specific feature in rotator cuff disease patients with hypercholesterolemia.

Keywords

Acknowledgement

Financial support: This work was supported by Biomedical Research Institute grant, Kyungpook National University Hospital (2021).

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