Journal of Applied Biological Chemistry

  • 제65권3호
  • /
  • Pages.153-158
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  • 2022
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UV

  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Hak-Dong (Department of Plant Science and Technology, Chung-Ang University) ;
  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • 투고 : 2022.06.17
  • 심사 : 2022.07.19
  • 발행 : 2022.09.30
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초록

This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using 1H-, 13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries.

키워드

과제정보

This research was supported by a grant from Natural Product Institute of Science and Technology, Anseong 17546, Republic of Korea. We gratefully acknowledge Mr. Dong-gun Woo for the collection of the plant material and fundamental side experiments.

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