The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Application of Bacterial Endophytes to Control Bacterial Leaf Blight Disease and Promote Rice Growth

  • Ooi, Ying Shing (School of Biological Sciences, Universiti Sains Malaysia) ;
  • Nor, Nik M.I. Mohamed (School of Biological Sciences, Universiti Sains Malaysia) ;
  • Furusawa, Go (Centre for Chemical Biology, Sains@USM, Universiti Sains Malaysia) ;
  • Tharek, Munirah (Soil Science, Water and Fertilizer Research Centre, MARDI) ;
  • Ghazali, Amir H. (School of Biological Sciences, Universiti Sains Malaysia)
  • 투고 : 2022.01.26
  • 심사 : 2022.08.09
  • 발행 : 2022.10.01
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초록

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) disease in rice (Oryza sativa L.) and it is among the most destructive pathogen responsible for severe yield losses. Potential bacterial biocontrol agents (BCAs) with plant growth promotion (PGP) abilities can be applied to better manage the BLB disease and increase crop yield, compared to current conventional practices. Thus, this study aimed to isolate, screen, and identify potential BCAs with PGP abilities. Isolation of the BCAs was performed from internal plant tissues and rhizosphere soil of healthy and Xoo-infected rice. A total of 18 bacterial strains were successfully screened for in vitro antagonistic ability against Xoo, siderophore production and PGP potentials. Among the bacterial strains, 3 endophytes, Bacillus sp. strain USML8, Bacillus sp. strain USML9, and Bacillus sp. strain USMR1 which were isolated from diseased plants harbored the BCA traits and significantly reduced leaf blight severity of rice. Simultaneously, the endophytic BCAs also possessed plant growth promoting traits and were able to enhance rice growth. Application of the selected endophytes (BCAs-PGP) at the early growth stage of rice exhibited potential in suppressing BLB disease and promoting rice growth.

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과제정보

The authors sincerely thank Universiti Sains Malaysia (USM) for research facilities and the Malaysian Agricultural Research and Development Institute (MARDI) for granting permission to access the study site and providing the rice seeds. The authors also greatly acknowledge Dr. Zarul Hazrin Hashim and Mr. Talha of the School of Biological Sciences, USM, for their assistance in the statistical data analysis.

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