The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Lysinabacillus fusiformis and Paenibacillus alvei Obtained from the Internal of NasutitermesTermites Revealed Their Ability as Antagonist of Plant Pathogenic Fungi

  • Fitriana, Yuyun (Department of Plant Protection, Faculty of Agriculture, University of Lampung) ;
  • Tampubolon, Desi Apriani Teresa (Department of Agrotechnology, Faculty of Agriculture, University of Lampung) ;
  • Suharjo, Radix (Department of Plant Protection, Faculty of Agriculture, University of Lampung) ;
  • Lestari, Puji (Department of Plant Protection, Faculty of Agriculture, University of Lampung) ;
  • Swibawa, I Gede (Department of Plant Protection, Faculty of Agriculture, University of Lampung)
  • Received : 2022.03.07
  • Accepted : 2022.07.17
  • Published : 2022.10.01
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Abstract

This study was performed to reveal phenotypic characters and identity of symbiont bacteria of Nasutitermes as well as investigate their potential as antagonist of plant pathogenic fungi. Isolation of the symbiont bacteria was carried out from inside the heads and the bodies of soldier and worker termite which were collected from 3 locations of nests. Identification was performed using phenotypic test and sequence of 16S ribosomal DNA (16S rDNA). Antagonistic capability was investigated in the laboratory against 3 phytopathogenic fungi i.e., Phytophthora capsici, Ganoderma boninense, and Rigidoporus microporus. Totally, 39 bacterial isolates were obtained from inside the heads and the bodies of Nasutitermes. All the isolates showed capability to inhibit growth of P. capsici, however, 34 isolates showed capability to inhibit growth of G. boninense and 32 isolates showed capability to inhibit growth of R. microporus. Two bacterial strains (IK3.1P and 1B1.2P) which showed the highest percentage of inhibition were further identified based on their sequence of 16S rDNA. The result showed that 1K3.1P strain was placed in the group of type strain and reference strains of Lysinibacillus fusiformis meanwhile 1B1.2P strain was grouped within type strain and reference strains Paenibacillus alvei. The result of this study supply valuable information on the role of symbiont bacteria of Nasutitermes, which may support the development of the control method of the three above-mentioned phytopathogenic fungi.

Keywords

Acknowledgement

This work was supported by Daftar Isian Pelaksanaan Anggaran (DIPA) Badan Layanan Umum (BLU) University of Lampung through fundamental research grant No. 1604/UN26.21/PN/2021. We thanks to Faculty of Agriculture, University of Lampung for permitting us using research facilities during this study.

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