The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Skin Moisturizing Activity of Unripe Apple(Immature Fruit of Malus pumila) in Mice

풋사과 추출물의 피부 보습 효과

  • Park, Hye Rim (Dept. of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jae Kwang (Korean Medicine-Application Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jae Kyoung (CNS Pharm Korea Co. Ltd.) ;
  • Choi, Beom Rak (Nutracore Co. Ltd.) ;
  • Kim, Jong Dae (Dept. of Pulmonary Internal Medicine, Daegu Haany University Daegu Oriental Hospital, Daegu Haany University) ;
  • Ku, Sae Kwang (Dept. of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Jegal, Kyung Hwan (Dept. of Korean Medical Classics, College of Korean Medicine, Daegu Haany University)
  • 박혜림 (대구한의대학교 한의과대학 해부조직학교실) ;
  • 김재광 (한국한의학연구원 한의기술응용센터) ;
  • 이재경 (한국씨엔에스팜) ;
  • 최범락 (뉴트라코어) ;
  • 김종대 (대구한의대학교 대구한의대한방병원 폐계내과학교실) ;
  • 구세광 (대구한의대학교 한의과대학 해부조직학교실) ;
  • 제갈경환 (대구한의대학교 한의과대학 원전학교실)
  • Received : 2022.10.20
  • Accepted : 2022.11.15
  • Published : 2022.11.25
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Abstract

Objectives : Skin aging is generally characterized by wrinkles, sagging, loss of elasticity roughness, pigmentation and dryness. This changes is caused by reducing the elements constituting the extracellular matrix contributing to the physiological properties of the skin, such as collagen fiber, elastic fiber, and hyaluronic acid. Adequate skin hydration is important to maintain normal skin function and reduce skin aging. The present study is objective to observe skin moisturizing effects of Unripe apple(UA, Immature fruit of Malus pumila Mill) in vivo and its underlying molecular mechanisms. Methods : ICR mice were orally administerd UA(100, 200 and 400mg/kg/day) for 8 weeks, and skin water contents and the expression of transforming growth factor (TGF)-𝛽1, ceramide, hyaluronan and collagen type I(COL1) were measured in dorsal back skin of the mice. Gene expression of hyaluronan synthase(HAS1, HAS2, HAS3), collagen synthase(COL1A1, COL1A2) and TGF-𝛽1 were also determined by realtime RT-PCR. Results : Skin water contents and the expression of TGF-𝛽1, ceramide, COL1 and hyaluronan were significantly increased in UA group(100, 200 and 400mg/kg/day) compared to vehicle control. The mRNA expression of HAS isoform(HAS1, HAS2, HAS3), COL1A1, COL1A2, and TGF-𝛽1 were also significantly increased by UA. Conclusions : UA has skin moisturizing effects and enhancement activities in skin function related components(COL1, hyaluronan, ceramide and TGF-𝛽1). These results suggested that UA can be a developing candidate for developing alternative skin protective agent or functional food ingredient.

Keywords

References

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