Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Effect of Light Intensity on Cell Growth and Carotenoids Production in Chlamydomonas reinhardtii dZL

Chlamydomonas reinhardtii dZL 균주의 광도가 세포 생장과 카로티노이드 생산량에 미치는 영향 연구

  • Seong-Joo Hong (Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University) ;
  • Hyunwoo Kim (Department of Biological Engineering, Inha University) ;
  • Jiho Min (Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University) ;
  • Hanwool Park (Department of Biological Engineering, Inha University) ;
  • Z-Hun Kim (Huevergreenpharm Inc.) ;
  • Chang Soo Lee (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Eonseon Jin (Department of Life Science, Hanyang University) ;
  • Choul-Gyun Lee (Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University)
  • 홍성주 (인하대학교 산학융합 인터랙티브 바이오공정 혁신 교육연구단) ;
  • 김현우 (인하대학교 생명공학과) ;
  • 민지호 (인하대학교 산학융합 인터랙티브 바이오공정 혁신 교육연구단) ;
  • 박한울 (인하대학교 생명공학과) ;
  • 김지훈 ((주)휴에버그린팜) ;
  • 이창수 (국립낙동강생물자원관 미생물연구실) ;
  • 진언선 (한양대학교 생명과학과) ;
  • 이철균 (인하대학교 산학융합 인터랙티브 바이오공정 혁신 교육연구단)
  • Received : 2023.08.08
  • Accepted : 2023.11.22
  • Published : 2023.12.31
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Abstract

Microalgae, as photosynthetic organisms, possess the ability to produce a diverse array of bioactive compounds. This study focused on the transformant Chlamydomonas reinhardtii dZL and subjected it to cultivation under varying light intensities (60, 120, 180, and 240 µmol/m2/s). Our aim was to assess the impact of light intensity on both microalgal biomass and carotenoid production. The cultivation took place in 80 mL bubble column photobioreactors, specifically the Multi-cultivator. Notably, the culture exposed to 240 µmol/m2/s exhibited the most rapid cell growth, surpassing even the cell concentration achieved at 180 µmol/m2/s by day 8. A detailed analysis of the specific irradiance rate over time unequivocally revealed a sharp decline in growth rates when the rate fell below 2 × 10-10 µmol/cell/s. Although the culture with 60 µmol/m2/s yielded the highest carotenoid content (1.2% of dry weight), the culture exposed to 240 µmol/m2/s recorded the highest carotenoid concentration at 8.9 mg/L owing to its higher biomass. Our findings reveal the critical importance of maintaining a specific irradiance rate above 2 × 10-10 µmol/cell/s to enhance biomass and carotenoid productivity. This study lays the groundwork for defining optimal light intensity conditions applicable to mass culture systems, with the objective of augmenting C. reinhardtii biomass and optimizing carotenoid productivity.

Keywords

Acknowledgement

이 연구는 2022년도 교육부의 재원으로 한국연구재단의 기초연구사업(NRF-2022R1I1A1A01052953)과 환경부의 재원으로 국립낙동강생물자원관에서 지원(NNIBR202303113)을 받아 수행된 연구입니다.

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