Mycobiology

The Korean Society of Mycology (한국균학회)
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Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam

  • Kim Cuc Thi Nguyen (Institute of Biotechnology, Hue University) ;
  • Phuc Hung Truong (Faculty of Biotechnology, TNU- University of Sciences) ;
  • Cuong Tu Ho (Institute of Environmental Technology, Vietnam Academy of Science and Technology) ;
  • Cong Tuan Le (Department of Environmental Science, University of Sciences, Hue University) ;
  • Khoa Dang Tran (Faculty of Agronomy, University of Agriculture and Forestry, Hue University) ;
  • Tien Long Nguyen (Department of Educational Management, University of Agriculture and Forestry, Hue University) ;
  • Manh Tuan Nguyen (Institute of Life Science, Thai Nguyen University of Agriculture and Forestry) ;
  • Phu Van Nguyen (Institute of Biotechnology, Hue University)
  • Received : 2023.01.18
  • Accepted : 2023.10.19
  • Published : 2023.12.31
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Abstract

In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerate Cu2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3-7), and temperature (10-40 ℃). Dried biomass of GL1 was able to remove Cu2+ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 ℃, and incubation time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations.

Keywords

Acknowledgement

The authors thank Dr. Derek Wilkinson for the English editing and proofreading of the manuscript.

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