Mycobiology

  • 제51권6호
  • /
  • Pages.452-462
  • /
  • 2023
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Determining Potential Link between Environmental and Clinical Isolates of Cryptococcus neoformans/Cryptococcus gattii Species Complexes Using Phenotypic and Genotypic Characterisation

  • Kenosi Kebabonye (School of Health Allied Professions, Faculty of Health Sciences, University of Botswana) ;
  • Mosimanegape Jongman (Department of Biological Sciences, Faculty of Science, University of Botswana) ;
  • Daniel Loeto (Department of Biological Sciences, Faculty of Science, University of Botswana) ;
  • Sikhulile Moyo (School of Health Allied Professions, Faculty of Health Sciences, University of Botswana) ;
  • Wonderful Choga (Research Laboratory, Botswana Harvard AIDS Institute Partnership) ;
  • Ishmael Kasvosve (School of Health Allied Professions, Faculty of Health Sciences, University of Botswana)
  • 투고 : 2023.02.16
  • 심사 : 2023.10.10
  • 발행 : 2023.12.31
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초록

Opportunistic infections due to Cryptococcus neoformans and C. gattii species complexes continue to rise unabated among HIV/AIDS patients, despite improved antifungal therapies. Here, we collected a total of 20 environmental and 25 presumptive clinical cryptococcal isolates from cerebrospinal fluid (CSF) samples of 175 patients enrolled in an ongoing clinical trial Ambition 1 Project (Botswana-Harvard Partnership). Identity confirmation of the isolates was done using MALDI-TOF MS and PCR. We describe the diversity of the isolates by PCR fingerprinting and sequencing (Oxford Nanopore Technology) of the intergenic spacer region. Mating types of the isolates were determined by amplification of the MAT locus. We report an unusual prevalence of 42.1% of C. neoformans × C. deneoformans hybrids Serotype AD (n = 16), followed by 39.5% of C. neoformans Serotype A (n = 15), 5.3% of C. deneoformans, Serotype D (n = 2), 7.9% of C. gattii (n = 3), and 5.3% of C. tetragattii (n = 2) in 38 representative isolates that have been characterized. Mating type-specific PCR performed on 38 representative environmental and clinical isolates revealed that 16 (42.1%) were MATa/MAT𝛼 hybrids, 17 (44.7%) were MAT𝛼, and five (13.2%) possessed MATa mating type. We used conventional and NGS platforms to demonstrate a potential link between environmental and clinical isolates and lay a foundation to further describe mating patterns/history in Botswana.

키워드

과제정보

The authors would like to express sincere gratitude to Professor Joe Jarvis and Ms Kwana Lechile who availed clinical isolates from their Ambition 1 Project. The MALDI-TOF MS for species identification was performed in Prof Lise Korsten's laboratory in University of Pretoria, and the biotyper was acquired from the National Research Foundation (NRF) of South Africa. We would like to send sincere gratitude to Prof. Wieland Meyer (Molecular Mycology Research Laboratory, Westmead Hospital, Australia) as the reference isolates used in our study originated from their laboratory.

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