대한의생명과학회지 (Biomedical Science Letters)

  • 제29권4호
  • /
  • Pages.249-255
  • /
  • 2023
  • /
  • 1738-3226(pISSN)
  • /
  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Swab in Maintaining Survival Efficiency according to the CLSI M40-A2 Standard

  • Hyeokjin Kwon (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Myeongguk Jeong (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Yeeun Kim (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Yunhee Chang (Department of Biomedical Laboratory Science, Kyungnam College of Information & Technology) ;
  • Myeonggi Jeong (Korea Standard Co., Ltd) ;
  • Go-Eun Choi (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • 투고 : 2023.11.09
  • 심사 : 2023.12.01
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Transporting clinical samples for microbiological testing requires a proper transport medium that guarantees the survival of microorganisms. Therefore, the aim of the study was to determine the ability of Amies Transport Medium (ATM) to maintain the viability of microorganisms in clinical specimens and its suitability as a transport medium for microbiological testing. This study evaluated the performance of swab provided by KS Co., Ltd. for three groups of bacteria comprising aerobic and facultative anaerobic bacteria, anaerobic bacteria, and fastidious bacteria, according to the Clinical and Laboratory Standard Institute (CLSI) 8.11.2. The ATM stability test was conducted by dividing the medium into two groups based on the product expiration date of use. All tested media, A and B (the date of manufacture and expiration date are different) showed ≥5 CFU, and there was no significant difference in the result values of Category A and Category B with different serial numbers for each test. The results of this experiment when cross-checked with the guidelines suggest that ATM is a suitable transport medium for microbiological testing, as it maintains the viability of microorganisms and is suitable for overgrowth trials. In addition, compared to the number of CFUs at the origin, the number of CFUs did not increase by more than 1 log after storage. These results have important implications for the development of transport media that can guarantee the survival of microorganisms in clinical specimens.

키워드

과제정보

This research was supported by the BB21plus funded by Busan Metropolitan City and Busan Techno Park.

참고문헌

  1. Avolio M, Camporese A. Liquid based microbiological transport systems: Conformity assessment of two commercial devices. Journal of Microbiological Methods. 2015. 115: 42-44. https://doi.org/10.1016/j.mimet.2015.05.004
  2. Drake C, Barenfanger J, Lawhorn J, Verhulst S. Comparison of easy-flow copan liquid stuart's and starplex swab transport systems for recovery of fastidious aerobic bacteria. Journal of Clinical Microbiology. 2005. 43: 1301-1303. https://doi.org/10.1128/JCM.43.3.1301-1303.2005
  3. Farhat SE, Thibault M, Devlin R. Efficacy of a swab transport system in maintaining viability of neisseria gonorrhoeae and streptococcus pneumoniae. Journal of Clinical Microbiology. 2001. 39: 2958-2960. https://doi.org/10.1128/JCM.39.8.2958-2960.2001
  4. Gizzie N, Adukwu E. Evaluation of liquid-based swab transport systems against the new approved clsi m40-a2 standard. Journal of Clinical Microbiology. 2016. 54: 1152-1156. https://doi.org/10.1128/JCM.03337-15
  5. Hindiyeh M, Acevedo V, Carroll KC. Comparison of three transport systems (starplex starswab ii, the new copan vi-pak amies agar gel collection and transport swabs, and bbl port-a-cul) for maintenance of anaerobic and fastidious aerobic organisms. Journal of Clinical Microbiology. 2001. 39: 377-380. https://doi.org/10.1128/JCM.39.1.377-380.2001
  6. Namekar M, Ellis EM, O'Connell M, Elm J, Gurary A, Park SY, Imrie A, Nerurkar VR. Evaluation of a new commercially available immunoglobulin m capture enzyme-linked immunosorbent assay for diagnosis of dengue virus infection. Journal of Clinical Microbiology. 2013. 51: 3102-3106. https://doi.org/10.1128/JCM.00351-13
  7. Perry JL. Assessment of swab transport systems for aerobic and anaerobic organism recovery. Journal of Clinical Microbiology. 1997. 35: 1269-1271. https://doi.org/10.1128/jcm.35.5.1269-1271.1997
  8. Rishmawi N, Ghneim R, Kattan R, Ghneim R, Zoughbi M, AbuDiab A, Turkuman S, Dauodi R, Shomali I, Issa AE-R. Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems. Journal of Clinical Microbiology. 2007. 45: 1278-1283. https://doi.org/10.1128/JCM.02110-06
  9. Tyrrell KL, Citron DM, Leoncio ES, Goldstein EJ. Comparison of the copan eswab system with an agar swab transport system for maintenance of fastidious anaerobic bacterium viability. Journal of Clinical Microbiology. 2016. 54: 1364-1367. https://doi.org/10.1128/JCM.03246-15
  10. Van Horn KG, Audette CD, Sebeck D, Tucker KA. Comparison of the copan eswab system with two amies agar swab transport systems for maintenance of microorganism viability. Journal of Clinical Microbiology. 2008. 46: 1655-1658. https://doi.org/10.1128/JCM.02047-07
  11. Wayne P. Clinical and laboratory standards institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100-S20. 2010.