Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Development of an Automatic PCR System Combined with Magnetic Bead-based Viral RNA Concentration and Extraction

  • MinJi Choi (Department of Senior Healthcare, Eulji University) ;
  • Won Chang Cho (Department of Research Institute of Bitvalue, Guro Digital G Valley Complex) ;
  • Seung Wook Chung (Department of Research Institute of Bitvalue, Guro Digital G Valley Complex) ;
  • Daehong Kim (Department of Radiological Science, College of Health Science, Eulji University) ;
  • Il-Hoon Cho (Department of Senior Healthcare, Eulji University)
  • Received : 2023.12.01
  • Accepted : 2023.12.08
  • Published : 2023.12.31
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Abstract

Human respiratory viral infections such as COVID-19 are highly contagious, so continuous management of airborne viruses is essential. In particular, indoor air monitoring is necessary because the risk of infection increases in poorly ventilated indoors. However, the current method of detecting airborne viruses requires a lot of time from sample collection to confirmation of results. In this study, we proposed a system that can monitor airborne viruses in real time to solve the deficiency of the present method. Air samples were collected in liquid form through a bio sampler, in which case the virus is present in low concentrations. To detect viruses from low-concentration samples, viral RNA was concentrated and extracted using silica-magnetic beads. RNA binds to silica under certain conditions, and by repeating this binding reaction, bulk samples collected from the air can be concentrated. After concentration and extraction, viral RNA is specifically detected through real-time qPCR (quantitative polymerase chain reaction). In addition, based on liquid handling technology, we have developed an automatic machine that automatically performs the entire testing process and can be easily used even by non-experts. To evaluate the system, we performed air sample collection and automated testing using bacteriophage MS2 as a model virus. As a result, the air-collected samples concentrated by 45 times then initial volume, and the detection sensitivity of PCR also confirmed a corresponding improvement.

Keywords

Acknowledgement

This work was supported by the Technology development Program (S3194404) funded by the Ministry of SMEs and Startups (MSS, Korea).

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