Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Relieving effect for respiratory inflammation of Gumiganghwal-tang

구미강활탕(九味羌活湯)의 호흡기 염증 완화효과

  • Bo-In Kwon (Research Institute of Korean Medicine, Sangji University) ;
  • Joo-Hee Kim (Research Institute of Korean Medicine, Sangji University)
  • 권보인 (상지대학교 한의학연구소) ;
  • 김주희 (상지대학교 한의학연구소)
  • Received : 2023.12.03
  • Accepted : 2023.12.17
  • Published : 2023.12.31
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Abstract

Objectives : Gumiganghwal-tang and its main components have been used for treatment of cough, headache, joint pain and fever. Using a respiratory inflammatory model, we intend to demonstrate the its anti-inflammatory effect and immune mechanism of Gumiganghwal-tang. Methods : We induced the respiratory inflammation mouse model by papain treatment. Female BALB/C mice (8 weeks old) were divided into three groups as follows: saline control group, papain treatment group (vehicle), papain and Gumiganghwal-tang (200 mg/kg) treatment group (n=4). To verify the anti-inflammatory effect of Gumiganghwal-tang extracts, we measured the infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) and nasal lavage fluid (NALF). Additionally, the efficacy of Gumiganghwal-tang extracts on Th2 cell population and alveolar macrophage in lung were analyzed by using flow cytometry. Results : Gumiganghwal-tang extracts administration decreased inflammatory cell infiltration in BALF and NALF, especially of eosinophils. Furthermore, interleukin-5 level was reduced in lung by drug administration. Interestingly, Gumiganghwal-tang extracts treatment also decreased the Th2 cell (CD4+GATA3+) population and increased the alveolar macrophage (CD11b+CD11c+) population in lung. Conclusions : Our findings indicate that Gumiganghwal-tang extracts have anti-inflammatory effects by mediating Th2 cell and alveolar macrophage cell activation.

Keywords

Acknowledgement

본 결과물은 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력 기반 지역혁신 사업의 결과입니다(2022RIS-005).

References

  1. Holgate, S.T. Innate and adaptive immune responses in asthma. Nature Medicine. 2012;18:673-683.
  2. Pawankar, R. Allergic diseases and asthma: a global public health concern and a call to action. World Allergy Organ. 2014.
  3. Cates, C.J., Jaeschke, R., Schmidt, S., Ferrer, M. Regular treatment with formoterol and inhaled steroids for chronic asthma: serious adverse events. Cochrane Database Systemic Review. 2013.
  4. Wener, R.R., Bel, E.H. Severe refractory asthma: an update. European Respiratory Review. 2013;22:227-235.
  5. Slader, C.A., Reddel, H.K., Jenkins, C.R., Armour, C.L., Bosnic-Anticevich, S.Z. Complementary and alternative medicine use in asthma: who is using what?. Respirology. 2006;11:373-387.
  6. Li, X.M. Treatment of asthma and food allergy with herbal intervention from traditional Chinise medicine. Mount Sinai Journal of Medicine: A Journal of Traditional and Personalized Medicine. 2011;78:697-716.
  7. Zhang, T., Srivastava, K., Wen, M.C., Yang, N., Cao, J., Busse, P., Brimingham, N., Goldfarb, J., Li, X.M. Pharmacology and immunological actions of a herbal medicine ASHMI on allergic asthma. Phytotherapy Research. 2010;24(7):1047-1055
  8. Lambrecht, B.N., Hanmmad, H. The immunology of asthma. Nature Immunology. 2015;16:45-56.
  9. Kim, H.Y., Dekruyff, R,H., Umetsu, D.T. The many paths to asthma: phenotype shaped by innate and adaptive immunity. Nature Immunology. 2010;11:577-584.
  10. Foster, P.S., Mould, A.W., Yang, M., Mackenzie, J., Mattes, J., Hogan, S.P., Mahalingam, S., Mackenzie, A.N., Rothenberg, M.E., Young, I.G., Matthaei, K.I., Webb, D.C. Elemental signals regulating eosinophil accumulation in the lung Immunological Reviews. 2001;179(1):173-181.
  11. Rosenberg, H.F., Dyer, K.D., Foster, P.S. Eosinophils: changing perspectives in health  and diseases. Nature Reviews Immunology. 2012;13:9-22.
  12. Isobe, Y., Kato, T., Arita, M. Emerging roles of eosinophils and eosinophil-derived lipid mediators in the resolution of inflammation. Frontiers in Immunology. 2012;3:270.
  13. Kato, M., Suzuki, M., Hayashi, Y., Kimura, H. Role of eosinophils and their clinical significance in allergic inflammation. Experimental Review Clinical Immunology. 2006;2:121-133.
  14. Uhm, T.G., Kim, B.S., Chung, I.Y. Eosinophil development, regulation of eosinophil-specific genes, and role of eosinophils in the pathogenesis of asthma. Allergy, Asthma Immunology Research. 2012;4:68-79.
  15. Kang, D., Kim, J.E. Fine, ultrafine, and yellow dust: emerging health problems in Korea. Journal of Korean Medical Science. 2014;29 (5):621-622.
  16. Kim, H.-S., Kim, D.-S., Yi, S.-M. Relationship between mortality and fine particles during Asian dust, smog-Asian dust, and smog days in Korea. International Journal of Environmental Health Research. 2012;22(6):518-530.
  17. Walford, H.H., Doherty, T.A. Diagnosis and management of eosinophilic asthma: a US perspective. Journal of Asthma and Allergy. 2014;7:53-65.
  18. Kay, A.B. Allergy and allergic disease. First of two parts. New England Journal of Medicine. 2001;344(1):30-37.
  19. Rose Jr., C.E., Lannigan, J.A., Kim, P., Lee, J.J., Fu, S.M., Sung, S.S. Muring lung  eosinophil activation and chemokine production in allergic airway inflammation. Cell and Molecular Immunology. 2010;7:361-374.
  20. Chu, D.K., Jimenez-Saiz, R., Verschoor, C.P., Walker, T.D., Goncharova, S., Llop-Guevara, A., Shen, P., Gordon, M.E., Barra, N.G., Bassett, J.D., Kong, J., Fattouh, R., McCoy, K.D., Bowdish, D.M., Erjefalt, J.S., Pabst, O., Humbles, A.A., Kolbeck, R., Waserman, S., Jordana, M. Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo. Journal of Experimental Medicine. 2014;211(8):1657-1672.
  21. Lim, H.F., Nair, P. Efficacy and safety of reslizumab in patients with moderate to severe eosinophilic asthma. Expert Review of Respiratory Medicine. 2015;9(2):135-142.
  22. Saradna, A., Do, D.C., Fu, Q.-L., Gao, P. Macrophage polarization and allergic asthma. Translational Research. 2017;191:1-14.
  23. Jiang, Z., Zhu, L. Update on the role of alternatively activated macrophages in asthma. Journal of Asthma and Allergy. 2016;9:101-107.
  24. Song, X., Xie, S., Lu, K., Wang, C. Mesenchymal stem cells alleviate experimental asthma by inducing polarization of alveolar macrophages. Inflammation. 2015;38:483-492.
  25. Tan, H.Y., Wang, N., Li, S., Hong, M., Wang, X., Feng, Y. The Reactive Oxygen Species in Macrophage Polarization: Reflecting its dual role in progression and treatment of human disease. Oxidative Medicine and Cellular Longevity. 2016;2795090.
  26. Kwon, B.-I., Hong, S,C., Shin, K., Choi, E.-H., Hwang, J.-J., Lee, S.-H. Innate type2 Immuntiy is Associated with Eosinophilic Pleural Effusion in Primary Spontaneous Pneumothorax. American Journal of Respiratory and Critical Care Medicine. 2013;188:577-585.
  27. Kwon, B,-I., Kim, T.W., Shin, K., Kim, Y. H., Yuk, C.M., Yuk, J.-M., Shin, D.-M., Jo, E.-K., Lee, C.-H., Lee, S.-H. Enhanced Th2 cell differentiation and function in the absence of Nox2. Allergy. 2017;72(2):252-265.
  28. Shin, K., Kataru, R.P., Park, H.J., Kwon, B.-I., Kim, T.W., Hong, Y.K., Lee, S.-H. Th2 cells and their cytokines regulate formation and function of lymphatic vessels. Nature communications. 2017;DOI:10.1038/ncomms7196.