Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

즉석섭취식품에 존재하는 Salmonella spp.와 Listeria monocytogenes의 검출을 위한 SureTectTM와 표현형 및 유전자형 방법의 비교

  • Kye-Hwan Byun (Department of Food Science and Technology, Chung-Ang University) ;
  • Byoung Hu Kim (Department of Food Science and Technology, Chung-Ang University) ;
  • Ah Jin Cho (Department of Food Science and Technology, Chung-Ang University) ;
  • Eun Her (Department of Food Science and Technology, Chung-Ang University) ;
  • Sunghee Yoon (Bosung Scientific Co., Ltd.) ;
  • Taeik Kim (Microbiology Division, Thermo Fisher Scientific Korea) ;
  • Sang-Do Ha (Department of Food Science and Technology, Chung-Ang University)
  • Received : 2023.01.30
  • Accepted : 2023.04.05
  • Published : 2023.04.30
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Abstract

The objective of this study is to compare and assess the effectiveness of real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), and the selective agar plate method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, the detection performance of the three methods (RT-PCR [SureTectTM kit and PowerChekTM kit], LAMP [3M MDS], selective agar) were similar at 0-10, 10-50, 50-100, and 100- CFU/mL of Salmonella spp. and L. monocytogenes. We found that with RT-PCR, the Ct value of salad was significantly higher (p<0.05) than other RTE foods, indicating that fiber plays a critical role as an obstacle to the rapid detection of Salmonella spp. However, the Ct value displayed a mixed pattern according to the inoculation level of L. monocytogenes. The use of rapid detection kits and machines mostly depends on the user's choice, with accuracy, ease of use, and economy being the primary considerations. As an RT-PCR kit, SureTectTM and PowerChekTM showed high accuracy in detecting Salmonella spp. and L. monocytogenes in RTE foods, showing that they can replace the existing RT-PCR kits available. Additionally, LAMP also showed excellent detection performance, suggesting that it has the potential to be used as a food safety management tool.

본 연구에서는 real-time PCR(SureTectTM kit와 PowerChekTM kit), LAMP(3M MDS), 선택 배지를 이용하여 즉석섭취식품에 존재하는 Salmonella spp.와 L. monocytogenes의 검출 능력을 비교 및 평가하고 식품 매트릭스가 real-time PCR의 결과에 미치는 영향을 조사하였다. 4가지 서로 다른 농도로 접종된 식품을 동일한 증균배지를 이용하여 증균 후 세 가지 방법으로 검출한 결과, real-time PCR, LAMP, 선택 배지에서 모두 양성으로 검출되어 인위적으로 접종된 식품에서의 검출 성능은 동등한 것으로 나타났다. 또한, 식품 매트릭스가 real-time PCR의 신속 검출에 미치는 영향을 조사한 결과, Salmonella spp.의 검출에서 샐러드가 다른 식품에 비해 Ct value가 유의적으로 높은 것으로 나타나, 섬유질이 풍부한 식품에 존재하는 Salmonella spp.의 검출을 위해서는 충분한 균질화와 균체의 탈리, 그리고 효율적인 DNA의 증폭이 필요함을 알 수 있었다. 반면, L. monocytogenes의 검출은 식품 매트릭스마다 상이하며 혼합적인 양상을 보였다. 현재의 식품공전 규정에서 식품에 존재하는 식중독균의 신속 검출을 위한 장비와 시약의 사용은 대부분 사용자의 선택에 의존하고 있다. 본 실험에서 real-time PCR로 사용된 SureTectTM kit와 PowerChekTM kit는 기존 real-time PCR kit의 대체재로서 사용이 가능할 것으로 판단되며, 또한, LAMP도 우수한 검출 성능을 보였기에 식품안전 관리 수단으로 활용될 가능성이 있음을 시사하고 있다.

Keywords

Acknowledgement

This research was supported by a grant (21153MFDS605) from the Ministry of Food and Drug Safety in 2023

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