Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Protective effect of Allium ochotense Prokh. extract against ethanol-induced cytotoxicity

산마늘 추출물의 알코올 유도 세포독성에 대한 간 세포 보호 효과

  • Tae Yoon Kim (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jong Min Kim (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Hyo Lim Lee (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Min Ji Go (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung Gyum Joo (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ju Hui Kim (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Han Su Lee (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seon Jeong Sim (Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute) ;
  • Ho Jin Heo (Division of Applied Life Science (BK21) & Institute of Agriculture and Life Science, Gyeongsang National University)
  • 김태윤 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 김종민 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 이효림 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 고민지 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 주승겸 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 김주희 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 이한수 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원) ;
  • 심선정 (경상남도 산림환경연구원 산림연구과) ;
  • 허호진 (경상국립대학교 응용생명과학부(BK21) & 농업생명과학연구원)
  • Received : 2023.03.28
  • Accepted : 2023.05.16
  • Published : 2023.06.30
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Abstract

This study aimed to evaluate the antioxidant and hepatoprotective effects of aqueous and 60% ethanol extracts of Allium ochotense Prokh. against alcohol-induced cytotoxicity as well as on the activities of alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl, ferric reducing antioxidant power, and malondialdehyde assays, and found that both extracts exhibited considerable antioxidant activities. Additionally, both extracts showed synergistic effects on the activities of alcohol-metabolic enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde dehydrogenase. In addition, 2'-7'-dichlorodihydrofluorescein diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed that aqueous and 60% ethanol extracts reduced oxidative stress and increased cell viability. Moreover, both extracts regulated the expression of apoptosis-related proteins, namely B-cell lymphoma (BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In conclusion, aqueous and 60% ethanol extracts of A. ochotense Prokh. might be valuable functional materials derived from natural resources for the prevention of ethanol-induced cytotoxicity.

본 실험에서는 산마늘 추출물의 in vitro 항산화 및 in vitro 생리활성 효능평가를 진행하였다. 산마늘 추출물은 페놀성 화합물과 플라보노이드 화합물을 함유하고 있으며, ABTS, DPPH 라디칼 소거 활성 등을 나타내었다, 특히, 산마늘 추출물은 우수한 알코올 대사 관련 효소 활성을 나타내었으며 더불어, HepG2 세포에서 에탄올로 유도된 스트레스에 대해 세포 생존율을 유의적으로 증가시켰으며, 활성 산소종의 생성을 효과적으로 감소시켰다. 그리고 산마늘 물 및 60% 에탄올 추출물은 에탄올로 세포독성이 유도된 간세포에서 BCl-2, BAX 및 pro-caspase-3의 발현량을 개선함으로써 세포사멸을 억제하는 것으로 확인되었다.

Keywords

Acknowledgement

본 연구는 경상남도 산림환경연구원의 '산마늘 건강기능성 식품소재 개발 연구용역(과제번호-2022-04530001)'의 지원을 받아 수행된 연구 결과입니다.

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