Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Effect of replacement feed ingredients of Micropterus salmoides in exotic species

  • Min-Gi Han (Department of Stem Cells and Regenerative Technology, Konkuk University) ;
  • Ran Lee (Department of Stem Cells and Regenerative Technology, Konkuk University) ;
  • Hyun Jung Park (Department of Animal Biotechnology, Sangji University) ;
  • Kyung Hoon Lee (Research Institute, Bellys Co. Ltd) ;
  • Hyuk Song (Department of Stem Cells and Regenerative Technology, Konkuk University)
  • Received : 2023.08.01
  • Accepted : 2023.10.14
  • Published : 2023.12.31
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Abstract

Background: Largemouth bass (Micropterus salmoides) is introduced species that has caused aquatic ecology activity both in vitro and in vivo were investigated for the possibility of application of the bass extract as an alternative feed ingredient. Methods: The bass oil was extracted using a 1-L supercritical extractor, while the protein was extracted from 250 g of bass dry matter, which was dissolved in 1 mL of H2O at 50℃. Both oil and protein extracts were evaluated antioxidant activities and the level of DPPH radical scavenging assay and nitric oxide (NO) production assay with lipopolysaccharide response. Oral administration of 6.6 µL/g bass protein and 5.38 µL/g bass oil conducted for investigating serological and physiological effect. Results: DPPH radical scavenging showed similar radical scavenging ability of 50 µM of ascorbic acid at 200 ㎍ of protein and 10% of oil treatment. NO concentration was diminished by the treatment of bass oil. Oral administration of both bass oil and proteins to mice showed that the body weight increase rate of the bass oil treated group was significantly reduced by 1.55 g compared to the other groups. The number of white blood cells (WBC) was increased by 4.52 k/µL in the bass protein-treated group and 4.44 k/µL in the bass oil-treated group compared to the control group. However, the serum IgG level did not show a significant difference between the bass extract-treated groups and the control group. Conclusions: These studies demonstrate that both bass oil and proteins extracted from the bass not only provide excellent effects of antioxidant and immune activity but can also be used as functional food supplements.

Keywords

Acknowledgement

This study was supported by a grant from the Korea Institute of Marine Science & Technology Promotion (no. 20220381), Ministry of Oceans and Fisheries, Republic of Korea.

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