Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Polysaccharides from Panax ginseng promote intestinal epithelial cell migration through affecting the Ca2+ related regulators

  • Huibin, Zhu (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Jianhong, Cao (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Xinyi, Liang (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Meng, Luo (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Anrong, Wang (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Ling, Hu (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine) ;
  • Ruliu, Li (Pi-wei Institute, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine)
  • Received : 2022.02.07
  • Accepted : 2022.05.23
  • Published : 2023.01.02
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Abstract

Background and aim: Panax ginseng, a key herbal medicine of replenishing Qi and tonifying Spleen, is widely used in the treatment of gastrointestinal diseases in East Asia. In this study, we aim to investigate the potential effects and mechanisms of polysaccharides from P. ginseng (PGP) on intestinal mucosal restitution which is one of the crucial repair modalities during the recovery of mucosal injury controlled by the Ca2+ signaling. Methods: Rat model of intestinal mucosal injury was induced by indomethacin. The fractional cell migration was carried out by immunohistochemistry staining with BrdU. The morphological observations on intestinal mucosal injury were also performed. Intestinal epithelial cell (IEC-6) migration in vitro was conducted by scratch method. Western-blot was adopted to determine the expressions of PLC-𝛾1, Rac1, TRPC1, RhoA and Cav-1. Immunoprecipitation was used to evaluate the levels of Rac1/PLC-𝛾1, RhoA/TRPC1 and Cav-1/TRPC1. Results: The results showed that PGP effectively reduced the assessment of intestinal mucosal injury, reversed the inhibition of epithelial cell migration induced by Indomethacin, and increased the level of Ca2+ in intestinal mucosa in vivo. Moreover, PGP dramatically promoted IEC-6 cell migration, the expression of Ca2+ regulators (PLC-𝛾1, Rac1, TRPC1, Cav-1 and RhoA) as well as protein complexes (Rac1/PLC-𝛾1, Cav-1/TRPC1 and RhoA/TRPC1) in vitro. Conclusion: PGP increases the Ca2+ content in intestinal mucosa partly through controlling the regulators of Ca2+ mobilization, subsequently promotes intestinal epithelial cell migration, and then prevents intestinal mucosal injury induced by indomethacin.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation of China (No.81673940), the project of Guangzhou science and technology (No.20160701335) and First-class discipline construction major project of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine Planning (2020) No.62. We thank "LingNan medicial research center of Guangzhou university of Chinese medicine" for providing us with some experimental equipment during our research.

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