The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture on Anti-inflammatory Activities in Lipopolysaccharide-stimulated RAW 264.7 Cells

강황(薑黃) 계지(桂枝) 복합물이 RAW 264.7 세포에서 항염증 활성에 미치는 영향

  • Ji, Choi (Department of Herbal Food and Nutrition, Daegu Haany University) ;
  • Hae-Jin, Park (DHU Bio Convergence Testing Center) ;
  • Il-ha, Jeong (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Min Ju, Kim (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Mi-Rae, Shin (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Soo, Roh (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Soon-Ae, Park (Department of Foodcare YAKSUN, Daegu Haany University) ;
  • Mi-Lim, Kim (Department of Foodcare YAKSUN, Daegu Haany University)
  • 최지 (대구한의대학교 한방식품학과) ;
  • 박해진 (대구한의대학교 바이오융복합시험센터) ;
  • 정일하 (대구한의대학교 한의학과 본초약리학교실) ;
  • 김민주 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 신미래 (대구한의대학교 한의학과 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의학과 본초약리학교실) ;
  • 박순애 (대구한의대학교 푸드케어학부) ;
  • 김미림 (대구한의대학교 푸드케어학부)
  • Received : 2023.02.10
  • Accepted : 2023.03.25
  • Published : 2023.03.30
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Abstract

Objectives : A persistent inflammatory response can cause diseases such as fibrosis, cancer, and allergies. This study aimed to investigate the anti-inflammatory activity of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture (CCM) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Methods : The total polyphenol and flavonoid contents of CCM were confirmed through an in vitro experiment. Also, radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Hydroxyl were confirmed. Moreover, ferric reducing antioxidant power (FRAP) activity were confirmed. After, CCM (50, 100, and 200 ㎍/mL) were applied to 0.1 ㎍/mL LPS-stimulated RAW264.7 cells. The levels of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined. Also, the expressions of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways were detected using Western blot. Results : As a result of in vitro experiments, there was an excellent antioxidant activity in CCM-treated cells. In addition, in RAW264.7 cells stimulated with LPS, the increased NO level was inhibited in a concentration-dependent manner by the treatment of CCM. In addition, inflammatory cytokines production were significantly inhibited in a concentration-dependent manner in CCM-treated group. CCM treatment significantly decreased the protein expressions of MAPKs. Moreover, the expressions of NF-κBp65 and cyclooxygenase-2 (COX-2) were significantly decreased when 200 mg/kg of CCM was applied, and phospho-inhibitor of nuclear factor kappa B-α (p-IκBα) and inducible nitric oxide synthase (iNOS) were significantly decreased at all concentrations treated with CCM. Conclusion : Our findings show that CCM exhibited excellent antioxidant activity and exhibited superior anti-inflammatory effect through the MAPKs and NF-κB pathways in LPS-stimulated RAW 264.7 macrophages.

Keywords

Acknowledgement

본 성과물은 농촌진흥청 연구사업 (세부과제번호: PJ01425103)의 지원에 의해 수행되었습니다.

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