한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제27권4호
  • /
  • Pages.185-193
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  • 2023
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Downregulation of the Expression of Steroidogenic Acute Regulatory Protein and Aromatase in Steroidogenic KGN Human Granulosa Cells after Exposure to Bisphenol A

  • Ji-Eun Park (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University) ;
  • Seung Gee Lee (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University) ;
  • Seung-Jin Lee (Developmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology) ;
  • Wook-Joon Yu (Developmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology) ;
  • Jong-Min Kim (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
  • 투고 : 2023.09.17
  • 심사 : 2023.11.24
  • 발행 : 2023.12.31
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초록

Although increasing evidence of cause-and-effect relationship between BPA exposure and female reproductive disorders have been suggested through many studies, the precise biochemical and molecular mechanism(s) by which BPA interferes with steroidogenesis in the ovarian cells still remain unclear. Therefore, the purpose of this study was to discover the steroidogenic biomarker(s) associated with BPA treatment in human granulosa cell line, KGN. In this study, our results obtained via the analysis of steroidogenesis-related protein expression in KGN cells using quantitative polymerase chain reaction (qPCR) and western blot analyses revealed that the expression levels of steroidogenic acute regulatory (StAR) and aromatase decreased considerably and gradually after BPA treatment in a dose-dependent manner under BPA treatment. Further, remarkable decreases in their expression levels at the cellular levels were also confirmed via immunocytochemistry, and subsequent StAR and aromatase mRNA expression levels showed profiles similar to those observed for their proteins, i.e., both StAR and aromatase mRNA expression levels were significantly decreased under BPA treatment at concentrations ≥0.1 μM. We observed that follicle stimulating hormone upregulated StAR and aromatase protein expression levels; however, this effect was suppressed in the presence of BPA. Regarding the steroidogenic effects of BPA on KGN cells, controversies remain regarding the ultimate outcomes. Nevertheless, we believe that the results here presented imply that KGN cells have a good cellular and steroidogenic machinery for evaluating endocrine disruption. Therefore, StAR and aromatase could be stable and sensitive biomarkers in KGN cells for the cellular screening of the potential risk posed by exogenous and environmental chemicals to female reproductive (endocrine) function.

키워드

과제정보

This work was supported by Korea Environment Industry & Technology Institute(KEITI) through Technology Development Project for Safety Management of Household Chemical Products, funded by Korea Ministry of Environment (MOE) (RS-2020-KE000797).

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