The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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The Anti-Bacterial Activity and Anti-Inflammatory Effect of Ethanol Complex Extracts of Safflower and Mother Wort

사플라워와 마더워트 등 에탄올복합추출물의 항균활성과 항염증 효과

  • 김현경 (서원대학교 식품공학과) ;
  • 이윤기 (에스에스케이 주식회사) ;
  • 최수빈 (에스에스케이 주식회사) ;
  • 김도완 (중원대학교 식품산업과)
  • Received : 2022.12.29
  • Accepted : 2023.01.11
  • Published : 2023.01.31
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Abstract

we are investigated the anti-inflammatory effects of Safflower and Mother wort Ethanol Complex Extracts(SEC) on lipopolysaccharide (LPS)-activated RAW 264.7 cells. The results demonstrated that pretreatment of SEC(500㎍/mL) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by Safflower and Mother wort Ethanol Complex Extracts were observed in the following. Safflower and Mother wort Ethanol Complex Extracts inhibited the translocation of NF-κB from the cytosol to the nucleus via the suppression of IκB-α phosphorylation and also inhibited LPS-stimulated NF-κB transcriptional activity. These findings suggest that Safflower and Mother wort Ethanol Complex Extracts exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of Safflower and Mother wort Ethanol Complex Extracts. Therefore, Safflower and Mother wort Ethanol Complex Extracts could be regarded as a potential source of natural anti-inflammatory agents.

사플라워와 마더워트 등 에탄올복합추출물(SEC)을 lipopolysaccharide(LPS)로 유도된 마우스 대식세포주인 RAW264.7 세포와 마우스 폐포 대식세포주인 MH-S 세포에서 사플라워와 마더워트 등 에탄올복합추출물((SEC)의 항균과 항염증 활성 효과를 조사하였다. 그 결과 SEC(500 ㎍/mL)의 전처리 LPS 자극 세포에서 iNOS 단백질 및 염증성 사이토카인 mRNA 발현을 크게 억제하였다. 사플라워와 마더워트 등 에탄올복합추출물(SEC)에 의한 항염증활성 효과는 다음과 같이 관찰되었다. 사플라워와 마더워트 등 에탄올복합추출물(SEC)은 IκB-α 인산화의 억제를 통해 시토졸에서 핵으로의 NF-κB의 전좌를 억제하고 또한 LPS로 자극된 NF-κB 전사 활성을 억제하였다. 이러한 결과는 사플라워와 마더워트 등 에탄올복합추출물(SEC)이 항염증 작용을 발휘 하고 사플라워와 마더워트 등 에탄올복합추출물(SEC)의 잠재적인 치료 가치성 및 기본 메커니즘을 규명 할 수 있었다. 결론적으로 사플라워와 마더워트 등 에탄올복합추출물(GRP)이 다양한 항염증 활성성분 함유 및 천연 항염증제의 잠재적 공급원으로 활용 가능성이 있음을 시사 하였다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 중소기업기술정보진흥원의"지역특화산업육성+(R&D, S3262949)의 사업의 지원을 받아 수행된 연구 결과이므로 이에 감사 드립니다.

References

  1. H. K. Kim, "The functional effects of anti-microbial activity and anti-inflammatory seaweed polysaccharide extracts," The Journal of the Convergence on Culture Technology, Vol. 4(2), pp. 155-163, 2018. http://dx.doi.org/10.17703/JCCT.2018.4.2.155.
  2. H. K. Kim, "Evaluation of intestinal immunity activity by steam-Heat Treatment and fermentation of lactic acid bacterial of fruit and vegetable compex extracts containing red ginseng," The Journal of the Convergence on Culture Technology, Vol. 8(6), pp. 935-941, 2021. http://dx.doi.org/10.17703/JCCT.2022.8.6.935.
  3. H. J. Lee, H. S. Kim, S. T. Kim, D. Park, J. T. Hong, Y. B. Kim and S. S. Joo, "Anti-inflamma tory effects of hexane fraction from white rose flower extracts via inhibition of inflammatory repertoires," Biomol Ther, Vol. 19, pp. 331-335, 2011. http://dx.doi.org/10.4062/biomoither.2011.
  4. Y. W. Kim, R. J. Zhao, S. J. Park, J. R. Lee, I. J. Cho, C. H. Yang, S. G. Kim and S. C. Kim"Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-κ B-dependent iNOS and proinflammatory cytokines production", Brit. J. Pharmacol. Vol.154(1), pp. 165-173, 2008. http://dx.doi.org/10.1038/bjp.2008.79.Epub2008Mar10
  5. A. K. Lee, S. H. Sung, Y. C. Kim and S. G. Kim, "Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappa B alpha phosphorylation, C/EBP and AP-1 activation," Brit. JPharmacol Vol.139(1), pp. 11-20, 2003. http://dx.doi.org/10.1038/sj.bjp.0705231
  6. S. Ghosh and M. Ksarin, "Missing pieces in the NF-κB puzzle," Cell. Vol.109, pp. S81-S96, 2002. http://dx.doi.org/10.1016/s0092-8674(02)00703-1.
  7. J. H. Kim, D. H. Kim, S. H. Beak, H. J. Lee, M. R. Kim, H. J. Kwon and C. H. Lee, "Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down regulation of NF-κB and p38 MAP kinase activity in LPS stimulated RAW 264.7 cells," Biochem. Pharmacol., Vol. 71(8). pp. 1198-1205, 2006. http://dx.doi.org/10.1016/j.bcp.2005.12.031.
  8. S. Y. Yu, Y. J. Lee, S. N. Kang, S. K. Lee, J. Y. Jang, H. K. Lee, J. H. Lim and O. H. Lee, "Analysis of food Components of Carthamus Tinctorius L. Seed and its Antimicrobial Activity," The Korean Society of food Preservation., Vol. 20(2), pp. 227-233, 2013. http://dx.doi.org/10.11002/kjfp.2013.20.2.227.
  9. S. C. Park, H. M. Na, Y. H. Bai, C. Cho, C. S. Na, J. S. Kim and L. Wilson, Jr, "The effect of Leonurus sibiricus on Ulterine Activity," Korean J. Animal Reprod. Vol. 19(4). pp. 245-250. 1995.
  10. [10]I. K. Bae, H. Y. Min, A. R. Han, E. K. Seo and S. K. Lee, "Suppression of lipopolysaccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells," Eur. J. Pharmacol, Vol.513(3) ,pp. 237-242, 2005. http://dx.doi.org/10.1016/j.ejphar.2005.03.011.
  11. [11]C. Porta, P. Larghi, M. Rimoldi, M. G. Totaro, P. Allavena, A. Mantovani and A. Sica, "Cellular and molecular pathways linking inflammation and cancer," Immunobiology. Vol.214(9-10), pp. 761-777,2009. http://dx.doi.org/10.1016/j.imbio.2009.06.014.
  12. [12]L. Sautebin "Prostaglandins and nitric oxide as molecular targets for anti-inflammatory therapy," Fitoterapia. Vol.71, pp. S48-57, 2000. http://dx.doi.org/10.1016/s0367-326x(00)00181-7.