한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제33권1호
  • /
  • Pages.1-12
  • /
  • 2023
  • /
  • 1229-1854(pISSN)
  • /
  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
권호 표지
DOI QR코드

DOI QR Code

월비가출탕(越婢加朮湯)의 항산화 및 항염증 효과에 대한 연구

The Study on Anti-Oxidant and Anti-Inflammatory Effects of Wolbigachul-tang

  • 임승규 (동신대학교 한의과대학 한방재활의학교실) ;
  • 설재욱 (동신대학교 한의과대학 한방재활의학교실) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학교실) ;
  • 정재민 (동신대학교 한의과대학 한방재활의학교실) ;
  • 선윤권 (동신대학교 한의과대학 침구학교실) ;
  • 최진봉 (동신대학교 한의과대학 한방재활의학교실)
  • Seung-Kyu Im (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Jae-Uk Sul (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Seon-Jong Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Jae-Min Jeong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Yun-Gwon Seon (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Dongshin University) ;
  • Jin-Bong Choi (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
  • 투고 : 2022.12.16
  • 심사 : 2023.01.06
  • 발행 : 2023.01.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives This study was conducted to find out the antioxidant and inflammatory improvement effects of Wolbigachul-tang. Methods To evaluate the antioxidant and anti-inflammatory effects of wolbigachul-tang, the total polyphenol and flavonoid contents were measured. And radical scavenging function was measured. In addition reactive oxygen species, nitric oxide, cytokines productions were measured by treating wolbigachul-tang in the lipopolysaccharide-induced RAW264.7 cells. We also measured the gene expression of inducible nitric oxide synthase, cyclooxygenase-2, cytokines too. Results Radical scavenging experiments showed significant concentration-dependent scavenging capacity increase. The amount of reactive oxygen species, nitric oxide, cytokines production in the cell all showed a significant concentration-dependent decrease compared to the control group. The gene expression of inducible nitric oxide synthase, cyclooxygenase-2, and cytokines also showed a significant concentration-dependent decrease compared to the control group. Conclusions In conclusion, it is believed that wolbigachul-tang can be applied to various inflammatory diseases.

키워드

참고문헌

  1. Ji NR, Park KY. Stress-induced biphasic ethylene and ROS biosynthesis are synergistically interacted in cell damage. Journal of Plant Biotechnology. 2011;38(1):22-9.  https://doi.org/10.5010/JPB.2011.38.1.022
  2. Gabriele P, Natasha I, Mariapaola C, Giovanni P, Federica M, Vincenzo A, Francesco S, Domenica A, Alessandra B. Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity. 2017;2017:8416763.  https://doi.org/10.1155/2017/8416763
  3. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clinical and Experimental Immunology. 2007;147(2):227-35.  https://doi.org/10.1111/j.1365-2249.2006.03261.x
  4. Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: a review. Veterinary World. 2018;11(5):627-35.  https://doi.org/10.14202/vetworld.2018.627-635
  5. Jeong WJ, Kim CY. The neurotransmitter pathway of itching. Journal of Life Science. 2017;27(5):600-10.  https://doi.org/10.5352/JLS.2017.27.5.600
  6. Jeong RA, Yeon KK, Dong HC, In SL, Sonn SS, Jeong HG, Lee HY, Lee HR. WBCEx1 reduces feeding efficiency ratio and visceral obesity in obese mice induced by high fat diet. J Korean Med Rehabil. 2011;21(1):1-22. 
  7. National Oriental Medicine College Collaborative Textbook Compilation Committee. Herbalogy. 3th ed. Seoul:Young Lim Publishing. 2014:331-2. 
  8. Tracy RP. The five cardinal signs of inflammation: calor, dolor, rubor, tumor … and penuria. The Journals of Gerontology. 2006;61(10):1051-2.  https://doi.org/10.1093/gerona/61.10.1051
  9. Hu XS. Lecture of synopsis of golden chamber. 1st ed. Paju:FishWood. 2017:301-4. 
  10. Cho HC, Kim JK, Han JH, Kim JP, Ahn CS, Hong Y. The effect of propolis intake duration and concentration on free radical scavenging activity, peroxide value and fatigue variables induced by exercise. Physical Activity and Nutrition. 2006;10(1):27-35. 
  11. Yeon SH, Ham HM, Sung JH, Kim YH, Namkoong SG, Jeong HS, Lee JS. Antioxidant activities of hot water extract from Cornus walteri Wanger against oxidative stress induced by tert-Butyl hydroperoxide in HepG2 cells. Journal of the Korean Society of Food Science and Nutrition. 2013;42(10):1525-32.  https://doi.org/10.3746/JKFN.2013.42.10.1525
  12. Hong GG. A study on skin aging caused by free-radical and on efficacy of antioxidant vitamins. Korean Journal of Aesthetics and Cosmetics Society. 2009;2(18):51-62. 
  13. Yoon HJ, Park YS. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. Journal of the Korean Society of Food Science and Nutrition. 2010;39(2):219-26.  https://doi.org/10.3746/JKFN.2010.39.2.219
  14. Kim MG, Oh MS, Jeon JS, Kim HT, Yoon MH. A study on antioxidant activity and antioxidant compound content by the types of tea. Journal of Food Hygiene and Safety. 2016;31(2):132-9.  https://doi.org/10.13103/JFHS.2016.31.2.132
  15. Lee JE, Park SY. Effects of flavonoids on skin according to their structural characteristics: a review. Asian Journal of Beauty and Cosmetology. 2022;20(1):133-43.  https://doi.org/10.20402/ajbc.2022.0006
  16. Lee SM, You YH, Kim KM, Park JJ, Jeong CS, Jhon DY, Ju WJ. Antioxidant activities of native Gwangyang Rubus coreanus Miq. Journal of the Korean Society of Food Science and Nutrition. 2012;43(3):327-32.  https://doi.org/10.3746/jkfn.2012.41.3.327
  17. Jung HH, Cho UM, Hwang HS, Cho K, Lee SR, Kim MS. Effect of steviol β-glucopyranosyl ester on the production of nitric oxide and inflammatory cytokines in RAW 264.7 cells. Journal of the Society of Cosmetic Scientists of Korea. 2018;44(3):239-47.  https://doi.org/10.15230/SCSK.2018.44.3.239
  18. Kim TY, Leem KH. Effect of cheongpyesagan-tang on LPS induced inflammation in RAW 264.7 cells. Journal of Physiology & Pathology in Korean Medicine. 2019;33(1):31-8.  https://doi.org/10.15188/kjopp.2019.02.33.1.31
  19. Lim JW, Kim HY, Kim KH. Nuclear factor-κB regulates cyclooxygenase-2 expression and cell proliferation in human gastric cancer cells. Laboratory Investigation. 2001;81(3):349-60.  https://doi.org/10.1038/labinvest.3780243
  20. Yim CY. Nitric oxide and cancer. The Korean Journal of Medicine. 2010;78(4):450-6. 
  21. Kim CM, Park YK. The effects of different extracts of Ostericum koreanum on the production of inflammatory mediators in LPS-stimulated RAW264.7 cells. The Korea Journal of Herbology. 2009;24(1):169-78. https://doi.org/10.6116/KJH.2009.24.1.169