Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Atopic Activities of Sargassum horneri Hot Water Extracts in 2,4-Dinitrochlorobezene-Induced Mouse Models

  • Ga-Eun Woo (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Hye-Ji Hwang (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • A-Yeoung Park (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Ji-Yoon Sim (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Seon-Young Woo (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Min-Ji Kim (Department of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • So-Mi Jeong (Institute of Fisheries Sciences, Pukyong National University) ;
  • Nak-Yun Sung (Division of Natural Product Research, Korea Prime Pharmacy CO., LTD.) ;
  • Dong-Sub Kim (Division of Natural Product Research, Korea Prime Pharmacy CO., LTD.) ;
  • Dong-Hyun Ahn (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
  • Received : 2022.11.04
  • Accepted : 2022.12.18
  • Published : 2023.03.28
Download PDF
⟨ Previous Next ⟩

Abstract

Atopic dermatitis (AD) is a chronic inflammation associated with skin hypersensitivity caused by environmental factors. The objent of this study was to assess the hot water extracts of Sargassum horneri (SHHWE) on AD. AD was induced by spreading 2,4-dinitrochlorobenzene (DNCB) on the BALB/c mice. The efficacy of SHHWE was tested by observing the immunoglobulin E (IgE), cytokine, skin clinical severity score and cytokine secretions in concanavalin A (Con A)-stimulated splenocytes. The levels of interleukine (IL)-4, IL-5 and IgE, the pro-inflammatory cytokines that are closely related, were notably suppressed in a does-dependent manner by SHHWE, whereas the level of interferon γ (IFN-γ), the atopy-related Th1 cytokine inhibiting the production of Th2 cytokines, was increased. Therefore, these results show that SHHWE has a potent anti- inhibitory effect on AD and is highly valuable for cosmetic development.

Keywords

Acknowledgement

This research was supported by Korea Institute of Marine Science & Technology Promotion(KIMST) funded by the Ministry of Oceans and Fisheries(20210656).

References

  1. Leung DY, Boguniewicz M, Howell MD, Nomura I, Hamid QA. 2004. New insights into atopic dermatitis. J. Clin. Invest. 113: 651-657. https://doi.org/10.1172/JCI21060
  2. Boguniewicz M, Leung DY. 2011. Atopic dermatitis: a disease of altered skin barrier and immune dysregulation. Immunol. Rev. 242: 233-246. https://doi.org/10.1111/j.1600-065X.2011.01027.x
  3. Beltrani VS. 1999 The clinical spectrum of atopic dermatitis. J. Allergy Clin. Immunol. 104: S87-S98. https://doi.org/10.1016/S0091-6749(99)70050-3
  4. Kabashima K. 2013. New concept of the pathogenesis of atopic dermatitis: interplay among the barrier, allergy, and pruritus as a trinity. J. Dermatol. Sci. 70: 3-11. https://doi.org/10.1016/j.jdermsci.2013.02.001
  5. Nam SH, Seo HW, Seo J, Park SH, Kim SB, Jung JS, et al. 2021. Purple corn extract alleviates 2, 4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice. Anim. Cells Syst. (Seoul) 25: 272-282. https://doi.org/10.1080/19768354.2021.1974938
  6. Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, et al. 2015. Anti-atopic activity of tuna heart ethanol extract. J. Korean Soc. Food Sci. Nutr. 44: 1-6. https://doi.org/10.3746/jkfn.2015.44.1.001
  7. Park YM. 2006. Advances in the pathophysiology of atopic dermatitis. Pediatr. Allergy Respir. Dis. 16:189-196.
  8. Kim TY. 2009. Treatment of atopic dermatitis: an update and review of the literature. Pediatr. Allergy Respir. Dis.19: 209-219.
  9. Brown ES, Allsopp PJ, Magee PJ, Gill CI, Nitecki S, Strain CR, et al. 2014. Seaweed and human health. Nutr. Rev. 72: 205-216. https://doi.org/10.1111/nure.12091
  10. Wen ZS, Xiang XW, Jin HX, Guo XY, Liu LJ, Huang YN, et al. 2016. Composition and anti-inflammatory effect of polysaccharides from Sargassum horneri in RAW264. 7 macrophages. Int. J. Biol. Macromol. 88: 403-413. https://doi.org/10.1016/j.ijbiomac.2016.02.025
  11. Koo JG, Jeong S. 2020. Isolation and characterization of polysaccharides purified from brown alga Sargassum horneri. Korean J. Fish. Aquat. Sci. 53: 681-687.
  12. Shao P, Chen X, Sun P. 2014. Chemical characterization, antioxidant and antitumor activity of sulfated polysaccharide from Sargassum horneri. Carbohydr. Polym. 105: 260-269. https://doi.org/10.1016/j.carbpol.2014.01.073
  13. Kim DS, Sung NY, Park SY, Kim G, Eom J, Yoo JG, et al. 2018. Immunomodulating activity of Sargassum horneri extracts in RAW264. 7 macrophages. J. Nutr. Health 51: 507-514. https://doi.org/10.4163/jnh.2018.51.6.507
  14. Suzuki R, Shimizu T, Kudo T, Ohtsuka Y, Yamashiro Y, Oshida K. 2002. Effects of n-3 polyunsaturated fatty acids on dermatitis in NC/Nga mice. Prostaglandins Leukot. Essent. Fatty Acids 66: 435-440. https://doi.org/10.1054/plef.2002.0370
  15. Tomimori Y, Tanaka Y, Goto M, Fukuda Y. 2005. Repeated topical challenge with chemical antigen elicits sustained dermatitis in NC/Nga mice in specific-pathogen-free condition. J. Invest. Dermatol. 124: 119-124. https://doi.org/10.1111/j.0022-202X.2004.23516.x
  16. Leung DY. 1999. Pathogenesis of atopic dermatitis. J. Allergy Clin. Immunol. 104: S99-S108. https://doi.org/10.1016/S0091-6749(99)70051-5
  17. Leung DY, Bieber T. 2003. Atopic dermatitis. Lancet 361: 151-160. https://doi.org/10.1016/S0140-6736(03)12193-9
  18. Rousset F, Robert J, Andary M, Bonnin JP, Souillet G, Chretien I, et al. 1991. Shifts in interleukin-4 and interferon-γ production by T cells of patients with elevated serum IgE levels and the modulatory effects of these lymphokines on spontaneous IgE synthesis. J. Allergy Clin. Immunol. 87: 58-69. https://doi.org/10.1016/0091-6749(91)90213-8
  19. Han SC, Yun JH, Yoon WJ, Kang HK, Yoo ES. 2022. Effect of Ficus erecta var. sieboldii (Miq.) king leaf extracts on mice immune cell activation. Yakhak Hoeji. 66: 26-33. https://doi.org/10.17480/psk.2022.66.1.26
  20. Grewe M, Bruijnzeel-Koomen CA, Schopf E, Thepen T, Langeveld-Wildschut AG, Ruzicka T, et al. 1998. A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis. Immunol. Today 19: 359-361. https://doi.org/10.1016/S0167-5699(98)01285-7
  21. Brandt EB, Sivaprasad U. 2011. Th2 cytokines and atopic dermatitis. J. Clin. Cell. Immunol. 2: 110-110
  22. Amin K. 2012. The role of mast cells in allergic inflammation. Respir. Med. 106: 9-14. https://doi.org/10.1016/j.rmed.2011.09.007
  23. Hashimoto Y, Takaoka A, Sugimoto M, Honma Y, Sakurai T, Futaki N, et al. 2011. Itch-associated scratching contributes to the development of dermatitis and hyperimmunoglobulinaemia E in NC/Nga mice. Exp. Dermatol. 20: 820-825. https://doi.org/10.1111/j.1600-0625.2011.01337.x
  24. Simon D, Braathen LR, Simon HU. 2004. Eosinophils and atopic dermatitis. Allergy 59: 561-570. https://doi.org/10.1111/j.1398-9995.2004.00476.x
  25. Agnello D, Lankford CS, Bream J, Morinobu A, Gadina M, O'Shea JJ, et al. 2003. Cytokines and transcription factors that regulate T helper cell differentiation: new players and new insights. J. Clin. Immunol. 23: 147-161. https://doi.org/10.1023/A:1023381027062
  26. Jeong DH, Ahn NK, Choi YU, Park JH, Bae NY, Park SH, et al. 2015. Inhibitory effect of Sargassum fulvellum water extract on 2, 4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in mice. Microbiol. Biotechnol. Lett. 43: 150-157. https://doi.org/10.4014/mbl.1504.04006
  27. Furue M, Terao H, Rikihisa W, Urabe K, Kinukawa N, Nose Y, et al. 2003. Clinical dose and adverse effects of topical steroids in daily management of atopic dermatitis. Br. J. Dermatol. 148: 128-133. https://doi.org/10.1046/j.1365-2133.2003.04934.x
  28. Kang BK, Kim MJ, Kim KB, Ahn DH. 2016. In vivo and in vitro inhibitory activity of an ethanolic extract of Sargassum fulvellum and its component grasshopper ketone on atopic dermatitis. Int. Immunopharmacol. 40: 176-183. https://doi.org/10.1016/j.intimp.2016.07.015
  29. Kawakami T, Ando T, Kimura M, Wilson BS, Kawakami Y. 2009. Mast cells in atopic dermatitis. Curr. Opin. Immunol. 21: 666-678. https://doi.org/10.1016/j.coi.2009.09.006
  30. Schreiber S, Kilgus O, Payer E, Kutil R, Elbe A, Mueller C, et al. 1992. Cytokine pattern of Langerhans cells isolated from murine epidermal cell cultures. J. Immunol. 149: 3524-3534. https://doi.org/10.4049/jimmunol.149.11.3525
  31. Tiegs G, Hentschel J, Wendel A. 1992. AT cell-dependent experimental liver injury in mice inducible by concanavalin A. J. Clin. Invest. 90: 196-203. https://doi.org/10.1172/JCI115836