Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of Anti-inflammatory and Antioxidant Abilities of Complex Extracts Produced from Leonurus japonicus Houtt., Houttuynia cordata Thunberg, and Citrus unshiu Markovich

익모초, 어성초, 진피 복합 추출물의 항염증 및 항산화 평가

  • Hun Gyu Lee (Department of Oriental Medicine Resources Graduate School of Joongbu University) ;
  • Joong-Gu Ji (Department of Herbal Health & Pharmacy, Joongbu University)
  • 이훈규 (중부대학교 대학원 한약자원학과) ;
  • 지중구 (중부대학교 한방보건제약학과)
  • Received : 2023.02.09
  • Accepted : 2023.02.21
  • Published : 2023.02.28
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Abstract

This study aimed to confirm the potential as a material for food, medicine, and cosmetics by measuring the physiological activity of the complex extract(Leonurus japonicus Houtt., Houttuynia cordata Thunberg and Citrus unshiu Markovich). The complex extract was with hot water, The evaluation of cytotoxicity, antioxidant, anti-inflammatory, and MAPKs of the extract was performed by using ELISA and western blot. Total polyphenols and flavonoid contents of complex extracts were 126.16 mg/g and 105.84 mg/g, respectively. The scavenging activities of DPPH and ABTS radical significantly increased in a dose-dependent in complex extracts. Compared to the control group, the complex extracts treatments significantly reduced concentrations of nitric oxide and inflammatory cytokine (i.e., IL-1β, TNF-α, and IL-6). Furthermore, the complex extracts treatments significantly reduced protein expression levels of MAPKs (i.e., ERK, JNK, and p38). The results indicate that the complex extract can be used for oxidative damage and inflammation. Thus, the results of this study can be used as basic data for composite materials for food, medicine, and cosmetics.

본 연구는 복합추출물(익모초, 어성초, 진피)의 생리활성을 측정하여 식·의약품, 화장품 등의 소재로서의 가능성을 확인하고자 하였다. 복합추출물은 열수 추출하였으며, 세포독성, 항산화, 항염증, MAPKs 등의 평가는 ELISA 및 western blot을 통해 확인하였다. 그 결과, 총 폴리페놀 함량은 35.53±0.48 mg GAE/g, 플라보노이드 함량은 8.18±0.16 mg QE/g으로 나타났으며, DPPH 와 ABTS radical 소거능은 농도 의존적으로 증가하였다. 대조군과 비교하여 세포독성은 400 ㎍/㎖ 농도까지 100% 이상의 생존율이 나타났으며, NO 및 염증성 사이토카인을 유의하게 감소시켰다. 또한, 복합추출물은 MAPKs(ERK, JNK, p38) 단백질 발현을 유의하게 억제시켰다. 이러한 결과는 복합추출물이 산화적 손상 및 염증에 사용될 수 있음을 나타낸다. 따라서 본 연구 결과는 식·의약품, 화장품용 복합소재의의 기초자료로 활용될 수 있을 것이다.

Keywords

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