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The Korean Society of Phycology (한국조류학회(藻類))
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Geminocystis urbisnovae sp. nov. (Chroococcales, Cyanobacteria): polyphasic description complemented with a survey of the family Geminocystaceae

  • Elena Polyakova (Institute of Fundamental Medicine and Biology, Kazan Federal University) ;
  • Svetlana Averina (Department of Microbiology, St. Petersburg State University) ;
  • Alexander Pinevich (Department of Microbiology, St. Petersburg State University)
  • Received : 2023.01.08
  • Accepted : 2023.06.12
  • Published : 2023.06.21
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Abstract

Progress in phylogenomic analysis has led to a considerable re-evaluation of former cyanobacterial system, with many new taxa being established at different nomenclatural levels. The family Geminocystaceae is among cyanobacterial taxa recently described on the basis of polyphasic approach. Within this family, there are six genera: Geminocystis, Cyanobacterium, Geminobacterium, Annamia, Picocyanobacterium, and Microcrocis. The genus Geminocystis previously encompassed two species: G. herdmanii and G. papuanica. Herein, a new species G. urbisnovae was proposed under the provision of the International Code of Nomenclature for algae, fungi, and plants (ICN). Polyphasic analysis was performed for five strains from the CALU culture collection (St. Petersburg State University, Russian Federation), and they were assigned to the genus Geminocystis in accordance with high 16S rRNA gene similarity to existing species, as well as because of proximity to these species on the phylogenetic trees reconstructed with RaxML and Bayes methods. Plausibility of their assignment to a separate species of the genus Geminocystis was substantiated with smaller cell size; stenohaline freshwater ecotype; capability to complementary chromatic adaptation of second type (CA2); distinct 16S rRNA gene clustering; sequences and folding of D1-D1' and B box domains of the 16S-23S internal transcribed spacer region. The second objective pursued by this communication was to provide a survey of the family Geminocystaceae. The overall assessment was that, despite attention of many researchers, this cyanobacterial family has been understudied and, especially in the case of the crucially important genus Cyanobacterium, taxonomically problematic.

Keywords

Acknowledgement

The authors thank O. Gavrilova for long-term collaboration, S. Smirnova for a supply of plankton samples, and N. Velichko for a help with transmission electron microscope. We thank St. Petersburg University Research Centers "Cultivation of Microorganisms", "Molecular and Cell Technologies", and "Chromas" for strains maintenance, analytical assistance, and genes sequencing. We especially thank the anonymous reviewers for their criticism and valuable suggestions that greatly helped us in upgrading and improvement of the manuscript.

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