Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bulb of Lilium longiflorum Thunb Extract Fermented with Lactobacillus acidophilus Reduces Inflammation in a Chronic Obstructive Pulmonary Disease Model

  • Ji-Eun Eom (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Gun-Dong Kim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Young In Kim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Kyung min Lim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Ju Hye Song (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Yiseul Kim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Hyeon-Ji Song (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Dong-Uk Shin (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Eun Yeong Lim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Ha-Jung Kim (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Sung Hoon Kim (BOTANOS) ;
  • Deuk Sik Lee (WellbeingLS) ;
  • So-Young Lee (Food Functionality Research Division, Korea Food Research Institute (KFRI)) ;
  • Hee Soon Shin (Food Functionality Research Division, Korea Food Research Institute (KFRI))
  • Received : 2023.01.13
  • Accepted : 2023.01.27
  • Published : 2023.05.28
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Abstract

Chronic obstructive pulmonary disease (COPD), one of the leading causes of death worldwide, is caused by repeated exposure to harmful matter, such as cigarette smoke. Although Lilium longiflorum Thunb (LLT) has anti-inflammatory effects, there is no report on the fermented LLT bulb extract regulating lung inflammation in COPD. Thus, we investigated the protective effect of LLT bulb extract fermented with Lactobacillus acidophilus 803 in COPD mouse models induced by cigarette smoke extract (CSE) and porcine pancreas elastase (PPE). Oral administration of the fermented product (LS803) suppressed the production of inflammatory mediators and the infiltration of immune cells involving neutrophils and macrophages, resulting in protective effects against lung damage. In addition, LS803 inhibited CSE- and LPS-induced IL-6 and IL-8 production in airway epithelial H292 cells as well as suppressed PMA-induced formation of neutrophil extracellular traps in HL-60 cells. In particular, LS803 significantly repressed the elevated IL-6 and MIP-2 production after CSE and LPS stimulation by suppressing the activity of the nuclear factor kappa-light-chain-enhancer of activated B (NFκB) in mouse peritoneal macrophages. Therefore, our results suggest that the fermented product LS803 is effective in preventing and alleviating lung inflammation.

Keywords

Acknowledgement

This research was financially supported by the Ministry of SMEs and Startups (MSS), Korea, under the "Regional Specialized Industry Development Program (R&D, S3074997)" supervised by the Korea Technology and Information Promotion Agency for SMEs (TIPA), and was supported by the Main Research Program (E0210202-02) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science and ICT.

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