Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Potential Probiotic Lactiplantibacillus plantarum LRCC5310 by Comparative Genomic Analysis and its Vitamin B6 Production Ability

  • Yunjeong Lee (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Nattira Jaikwang (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Seong keun Kim (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Jiseon Jeong (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Ampaitip Sukhoom (Division of Biological Science, Faculty of Science, Prince of Songkla University) ;
  • Jong-Hwa Kim (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Wonyong Kim (Department of Microbiology, Chung-Ang University College of Medicine)
  • Received : 2022.11.07
  • Accepted : 2023.01.26
  • Published : 2023.05.28
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Abstract

Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T , L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2021R1C1C2003223 and NRF-2022R1A2C2012209), and the Chung-Ang University Research Grants in 2022.

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