한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권2호
  • /
  • Pages.167-173
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  • 2023
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Action of Extracellular Protease of Aspergillus terreus on Human Plasma Hemostasis Proteins

  • Alexander A. Osmolovskiy (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University) ;
  • Elena S. Zvonareva (Higher School of Economics - National Research University) ;
  • Nina A. Baranova (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University) ;
  • Valeriana G. Kreyer (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University)
  • 투고 : 2022.05.06
  • 심사 : 2022.06.30
  • 발행 : 2023.06.28
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초록

Proteolytic enzymes secreted by Aspergillus, as pathogenicity factors, affect blood coagulation and fibrinolysis, and therefore the target proteins of their action in the bloodstream are of significant interest. In the present study, the action of the isolated protease of A. terreus 2 on different human plasma proteins was shown. The protease of A. terreus 2 exhibited the highest proteolytic activity against hemoglobin, which was 2.5 times higher than the albuminolytic activity shown in both of the protein substrates used. In addition, the protease has significant ability to hydrolyze both fibrin and fibrinogen. However, the inability of the A. terreus 2 protease to coagulate rabbit blood plasma and coagulate human and bovine fibrinogen indicates the severity of the enzyme's action on human blood coagulation factors. It should be considered as a potential indicator of this isolated protease's participation in fungal pathogenesis. The protease shows no hemolytic activity. Furthermore, its activity is insignificantly inhibited by thrombin inhibitors, and is not inhibited by plasmin inhibitors.

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과제정보

This work was supported by the Financial Council for Grants of the President of the Russian Federation No. SP-3906.2021.4.

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