한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권2호
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  • Pages.203-207
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  • 2023
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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옥수수 근권토양으로부터 N2O 환원 근권세균 Pseudomonas sp. M23의 분리 및 특성

Isolation and Characterization of a N2O-Reducing Rhizobacterium, Pseudomonas sp. M23 from Maize Rhizosphere Soil

  • 김지윤 (이화여자대학교 환경공학과) ;
  • 이수연 (이화여자대학교 환경공학과) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Ji-Yoon Kim (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Soo Yeon Lee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Kyung-Suk Cho (Department of Environmental Science and Engineering, Ewha Womans University)
  • 투고 : 2023.03.24
  • 심사 : 2023.05.19
  • 발행 : 2023.06.28
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초록

옥수수 근권 토양으로부터의 N2O 환원 근권세균인 Pseudomonas sp. M23을 분리하였다. M23 균주의 최대 N2O 환원속도는 15.6 mmol·g-dry cell-1·h-1이었다. M23 균주의 N2O 환원 활성은 디젤 오염물에 의해 저해받지 않았고, 옥수수와 톨페스큐 뿌리삼출물 첨가에 의해 향상되었다. M23 균주 접종은 옥수수와 톨페스큐를 이용한 디젤 오염 토양의 정화 효율을 저해하지 않았다. M23 균주를 접종한 토양에서 재배한 식물체의 뿌리무게는 미접종 토양에서의 뿌리무게보다 컸으나, 유의적 차이는 없었다. 이러한 결과는 Pseudomonas sp. M23이 유류 오염 토양의 근권정화 과정에서 N2O 배출을 저감하는데 활용 가능한 유용한 세균임을 시사한다.

The N2O-reducing rhizobacterium, Pseudomonas sp. M23, was isolated from maize rhizosphere soil. The maximum N2O reduction rate of the strain M23 was 15.6 mmol·g-dry cell weight-1·h-1. Its N2O reduction activity was not inhibited by diesel contaminant, and it was enhanced by the addition of the root exudates of maize and tall fescue. The remediation efficiency of diesel-contaminated soil planted with maize or tall fescue was not inhibited by inoculating with the strain M23. Root weights in the soil inoculated with the strain M23 were greater than those in the non-inoculated soil. These results suggest that Pseudomonas sp. M23 is a promising bacterium to mitigate N2O emissions during the remediation of diesel-contaminated soil.

키워드

과제정보

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government through the Ministry of Science and ICT (MSIT) (2019R1A2C2006701 & 2022R1A2C2006615).

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