Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15

  • Hao Ding (Department of Orthopedics, Jinling Hospital, Jinling Clinical Medical College of Nanjing Medical University) ;
  • Xiaoliang Mei (Department of Orthopedics, The Affiliated Taizhou People's Hospital of Nanjing Medical University) ;
  • Lintao Li (Department of Orthopedics, Jinling Hospital, Jinling Clinical Medical College of Nanjing Medical University) ;
  • Peng Fang (Department of Orthopedics, Jinling Hospital, Jinling Clinical Medical College of Nanjing Medical University) ;
  • Ting Guo (Department of Orthopedics, Jinling Hospital, Jinling Clinical Medical College of Nanjing Medical University) ;
  • Jianning Zhao (Department of Orthopedics, Jinling Hospital, Jinling Clinical Medical College of Nanjing Medical University)
  • Received : 2022.08.31
  • Accepted : 2022.11.26
  • Published : 2023.04.30
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Abstract

Leucine-rich repeat containing 15 (LRRC15) has been identified as a contributing factor for cartilage damage in osteoarthritis; however, its involvement in rheumatoid arthritis (RA) and the underlying mechanisms have not been well characterized. The purpose of this study was to explore the function of LRRC15 in RA-associated fibroblast-like synoviocytes (RA-FLS) and in mice with collagen-induced arthritis (CIA) and to dissect the epigenetic mechanisms involved. LRRC15 was overexpressed in the synovial tissues of patients with RA, and LRRC15 overexpression was associated with increased proliferative, migratory, invasive, and angiogenic capacities of RA-FLS and accelerated release of pro-inflammatory cytokines. LRRC15 knockdown significantly inhibited synovial proliferation and reduced bone invasion and destruction in CIA mice. Runt-related transcription factor 1 (RUNX1) transcriptionally represses LRRC15 by binding to core-binding factor subunit beta (CBF-β). Overexpression of RUNX1 significantly inhibited the invasive phenotype of RA-FLS and suppressed the expression of proinflammatory cytokines. Conversely, the effects of RUNX1 were significantly reversed after overexpression of LRRC15 or inhibition of RUNX1-CBF-β interactions. Therefore, we demonstrated that RUNX1-mediated transcriptional repression of LRRC15 inhibited the development of RA, which may have therapeutic effects for RA patients.

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References

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