대한약침학회지 (Journal of Pharmacopuncture)

  • 제26권2호
  • /
  • Pages.139-146
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  • 2023
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  • 2093-6966(pISSN)
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  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
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Antibacterial and Anti-Glucosyltransferase Activity of Verbascum speciosum Against Cariogenic Streptococci

  • Shabnam Pourmoslemi (Department of Pharmaceutics, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences) ;
  • Amir Larki-Harchegani (Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences) ;
  • Sajad Daneshyar (Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences) ;
  • Dara Dastan (Department of Pharmacognosy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences) ;
  • Amir Nili-Ahmadabadi (Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences) ;
  • Mina Jazaeri (Department of Oral Medicine, Hamadan Dental Faculty, Hamadan University of Medical Sciences)
  • 투고 : 2022.09.14
  • 심사 : 2023.03.16
  • 발행 : 2023.06.30
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초록

Objectives: Dental caries is a prevalent chronic human infection worldwide and several plants have shown anticariogenic properties through antibacterial activity against oral pathogens. The present study aimed to assess anticariogenic activity of Verbascum speciosum, in search of novel agents for the prevention and treatment of dental caries. Methods: Hydro-alcoholic extracts from flowers and total aerial parts of the plant were prepared by maceration. Antibacterial activity of the extracts against Streptococcus mutans (ATCC 35668) and Streptococcus sobrinus (ATCC 27607) was investigated by agar diffusion and microdilution techniques. Inhibitory concentration-fifty values of the flowers' extract against Streptococcus mutans glucosyltransferase enzymes were determined. The total flavonoid content of the extracts was determined using an aluminum chloride reaction. Results: Verbascum speciosum flowers' extract showed significantly higher flavonoid content and antibacterial activity; with minimum inhibitory concentrations of 100 and 200 ㎍/mL for Streptococcus mutans and Streptococcus sobrinus, respectively. The extract inhibited the synthesis of glucan by cell-associated and extracellular glucosyltransferase enzymes in a dose-dependent manner with higher activity against the extracellular enzyme. Conclusion: This study indicated effective anticariogenic activity of Verbascum speciosum flowers extract. This extract can be considered as an alternative to current anticaries therapies or an additive to dental care products.

키워드

과제정보

We would like to declare our special thanks to the Research Affairs of Hamedan University of Medical Sciences for financial support. The authors wish to thank Ms. Sahar Foroughinia for her excellent technical assistance.

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