대한약침학회지 (Journal of Pharmacopuncture)

  • 제26권2호
  • /
  • Pages.167-174
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  • 2023
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  • 2093-6966(pISSN)
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  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
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In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat

  • Mohammad Reza Ghasemi (Department of Pharmacology &Toxicology, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences) ;
  • Akram Ranjbar (Department of Pharmacology &Toxicology, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences) ;
  • Pari, Tamri (Department of Pharmacology &Toxicology, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences) ;
  • Shabnam Pourmoslemi (Department of Pharmaceutics, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences) ;
  • Alireza Nourian (Department of Laboratory Sciences, Faculty of Para-Veterinary Sciences, Bu-Ali Sina University) ;
  • Dara Dastan (Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences)
  • 투고 : 2023.01.07
  • 심사 : 2023.02.20
  • 발행 : 2023.06.30
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초록

Objectives: In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the broth dilution method. Methods: The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment. Results: Our results revealed the bacteriostatic activity of AMEO against S. aureus and P. aeruginosa. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group. Conclusion: The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.

키워드

과제정보

This study was adapted from a PharmD thesis at Hamadan University of Medical Sciences. The study was funded by Vicechancellor for Research and Technology, Hamadan University of Medical Sciences, Hamadan, Iran (grant No: 140003252548).

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