Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The oral microbiome of implant-abutment screw holes compared with the peri-implant sulcus and natural supragingival plaque in healthy individuals

  • MinKee Son (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Yuri Song (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Yeuni Yu (Interdisciplinary Program of Genomic Science, Pusan National University) ;
  • Si Yeong Kim (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Jung-Bo Huh (Dental Research Institute, BK21 PLUS Project, School of Dentistry, Pusan National University) ;
  • Eun-Bin Bae (Dental Research Institute, BK21 PLUS Project, School of Dentistry, Pusan National University) ;
  • Won-Tak Cho (Dental Research Institute, BK21 PLUS Project, School of Dentistry, Pusan National University) ;
  • Hee Sam Na (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Jin Chung (Department of Oral Microbiology, School of Dentistry, Pusan National University)
  • Received : 2022.12.15
  • Accepted : 2023.04.27
  • Published : 2023.06.30
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Abstract

Purpose: An implant-supported prosthesis consists of an implant fixture, an abutment, an internal screw that connects the abutment to the implant fixture, and the upper prosthesis. Numerous studies have investigated the microorganisms present on the implant surface, surrounding tissues, and the subgingival microflora associated with peri-implantitis. However, there is limited information regarding the microbiome within the internal screw space. In this study, microbial samples were collected from the supragingival surfaces of natural teeth, the peri-implant sulcus, and the implant-abutment screw hole, in order to characterize the microbiome of the internal screw space in healthy subjects. Methods: Samples were obtained from the supragingival region of natural teeth, the peri-implant sulcus, and the implant screw hole in 20 healthy subjects. DNA was extracted, and the V3-V4 region of the 16S ribosomal RNA was sequenced for microbiome analysis. Alpha diversity, beta diversity, linear discriminant analysis effect size (LEfSe), and network analysis were employed to compare the characteristics of the microbiomes. Results: We observed significant differences in beta diversity among the samples. Upon analyzing the significant taxa using LEfSe, the microbial composition of the implant-abutment screw hole's microbiome was found to be similar to that of the other sampling sites' microbiomes. Moreover, the microbiome network analysis revealed a unique network complexity in samples obtained from the implant screw hole compared to those from the other sampling sites. Conclusions: The bacterial composition of the biofilm collected from the implant-abutment screw hole exhibited significant differences compared to the supra-structure of the implant. Therefore, long-term monitoring and management of not only the peri-implant tissue but also the implant screw are necessary.

Keywords

Acknowledgement

This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science & ICT (NRF-2017M3A9B6062021).

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