Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Antioxidant, Anti-inflammatory and Cartilage Regeneration Effects of Euiiin-tang

의이인탕(薏苡仁湯)의 항산화, 항염증 및 연골재생 효과

  • Hong-Tak Park (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Young-Jun Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Woo-Seok Shon (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Chang-Hoon Woo (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University)
  • 박홍탁 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 김영준 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 손우석 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2023.04.26
  • Accepted : 2023.07.03
  • Published : 2023.07.31
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Abstract

Objectives The purpose of this study was to investigate the antioxidant, anti-inflammatory and cartilage regeneration effects of Euiiin-tang water extract (EIIT) in the treatment of monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Methods Animal models were divided into five groups. The normal group didn't do any treatments causing osteoarthritis. The control group was orally administerd distilled water instead of the drug, the positive control group used indomethacin 5 mg/kg, the EIIT 100 group used EIIT 100 mg/kg and the EIIT 200 group used EIIT 200 mg/kg, and seven rats were placed per group. We administered drug to rats for 2 weeks and analyzed oxidative stress-related proteins in joint tissue. Inflammation mediators and inflammatory cytokines induced by the activity of inflammation-related proteins were analyzed. In addition, the expression of anti-inflammatory cytokines and collagen-related factors were analyzed, and H&E staining and Safranin-O staining were performed to see the effect on histopathological changes. Results 1) Oxidative stress-related proteins were significantly reduced. 2) Inflammationrelated proteins, inflammatory mediators and inflammatory cytokines were significantly reduced. 3) Anti-inflammatory cytokines were significantly increased. 4) Collagen proteolysis factors significantly decreased, and collagen degradation inhibitory factor was significantly increased. 5) EIIT administration significantly reduced cartilage degeneration and deformation in H&E staining, and reduced proteoglycan destruction in Safranin-O staining. Conclusions From the above experimental results, it judges that Euiiin-tang has antioxidant, anti-inflammatory, and cartilage regeneration effects on osteoarthritis in rats induced by MIA.

Keywords

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