Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

  • Juyeon Lee (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Minkyoung Kang (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Jiseon Yoo (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Sujeong Lee (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Minji Kang (Department of Functional Food and Biotechnology, Jeonju University) ;
  • Bohyun Yun (Division of Practical Application, Honam National Institute of Biological Resources) ;
  • Jong Nam Kim (Department of Food Science and Nutrition, Dongseo University) ;
  • Hyoungsun Moon (EN Food Contents Inc.) ;
  • Yihyung Chung (Jeonbuk Institute for Food-Bioindustry) ;
  • Sangnam Oh (Department of Functional Food and Biotechnology, Jeonju University)
  • Received : 2023.03.02
  • Accepted : 2023.03.09
  • Published : 2023.07.28
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Abstract

Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.

Keywords

Acknowledgement

This work was supported by the Technological Innovation R&D Program (S3084485) funded by the Ministry of SMEs and Startups (MSS, Korea) and the National Research Foundation of Korea Grant, funded by the Korean government (MEST) (2021R1A2C3011051).

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