The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Comparison of Mycelium Cultivation and Fruiting Body Characteristics of Lentinula edodes According to the Sawdust Media Nutrients and Inoculation and Cultivation Conditions

표고 톱밥배지 영양원, 접종 및 배양조건에 따른 균사배양 및 자실체 특성 비교

  • Yeun Sug Jeong (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science)
  • 정연석 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 김민준 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 장영선 (국립산림과학원 산림생명자원연구부 산림미생물연구과) ;
  • 가강현 (국립산림과학원 산림생명자원연구부 산림미생물연구과)
  • Received : 2023.03.21
  • Accepted : 2023.06.05
  • Published : 2023.06.30
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Abstract

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

표고버섯의 배양조건은 버섯의 생산량과 품질에 많은 영향을 준다. 본 연구에서는 표고버섯의 배양조건을 변화시켜 생산성 및 품질을 개량하기 위한 연구를 수행하였다. 액체와 고체 두 가지 형태의 종균을 사용하였고, 톱밥배지의 영양원으로 옥수수가루를 사용하였다. 명배양 과정에서는 광조건을 청색광으로 변화시켜 연구를 수행하였다. 실험품종으로는 산백향을 이용하였다. 옥수수가루를 이용한 결과 배양 21일까지의 균사생장률이 최대 2.7배 빠른 것으로 나타났으며, 배지의 중량 감소율이 더 높은 것으로 나타났다. 액체종균을 사용한 실험군에서는 톱밥종균을 이용하였을 때 보다 자실체의 생산량이 1.2배 증가하는 것을 확인할 수 있었으며, 청색광을 이용한 실험군에서는 1.1배의 생산량이 증가하였다. 자실체의 무게와 갓 크기는 톱밥종균을 사용한 경우 더 높게 나타났으며, 버섯의 무게 및 대두께는 청색광을 이용한 조건에서 더 우월한 것으로 나타났다. 전자혀를 통한 맛 분석 결과 옥수수가루를 사용하였을 때 짠맛이 증가하였고, 청색광을 사용하면 시큼한 맛이 증가하는 것을 확인할 수 있었다.

Keywords

Acknowledgement

This study was supported by a grant from the General Project (FP0800-2023-01) of the National Institute of Forest Science, Republic of Korea.

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