한국치위생학회지 (Journal of Korean society of Dental Hygiene)

  • 제23권4호
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  • Pages.219-226
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  • 2023
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  • 2287-1705(pISSN)
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  • 2288-2294(eISSN)
한국치위생학회 (Korean Society of Dental Hygiene)
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대황 추출물이 골수유래 대식세포의 파골세포 분화에 미치는 영향

Effects of rhubarb extract on osteoclast differentiation in bone marrow-derived macrophages

  • In-A Cho (Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University)
  • 투고 : 2023.08.09
  • 심사 : 2023.08.21
  • 발행 : 2023.08.30
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초록

연구목적: 이 연구는 대황 추출물이 골수 유래 대식세포(BMM)에서 파골세포 분화에 미치는 영향을 조사하는 것을 목적으로 한다. 파골 세포는 골 재흡수 및 재형성에 중요한 역할을 하며, 파골 세포의 조절 장애는 다양한 골 관련 질환을 유발할 수 있다. 잠재적인 항염증 특성을 가진 약용 식물인 대황은 뼈 대사를 조절하는 것으로 제안되었다. 연구방법: 생후 5주령의 C57BL/6 마우스의 대퇴골과 경골에서 BMM을 분리하고 M-CSF(mouse macrophage colony-stimulating factor) 존재하에 3일간 배양한 후 M-CSF와 파골 세포 분화를 유도하기 위한 핵 인자-κB 리간드(RANKL)의 활성화제를 처리하였다. 연구결과: 대황 추출물로 처리하면 BMM에서 파골 세포 분화가 현저하게 억제되었다. 또한 대황 추출물은 파골세포 형성에 필수적인 유전자인 TRAP(tartrate-resistant acid phosphatase) 및 CTSK(cathepsin K)의 mRNA 발현을 억제하였다. 또한 파골세포 분화에 중요한 전사 인자인 활성화된 T 세포 c1(NFATc1)의 핵 인자의 RANKL 유도 발현을 억제하였다. 결론: 이러한 결과는 대황 추출물이 BMMs에서 파골 세포 형성에 억제 효과가 있음을 나타낸다. 따라서 대황 추출물은 비정상적인 파골 세포 활동과 관련된 뼈 관련 질환의 치료를 위한 유망한 치료제이다. 잠재적인 임상 적용을 완전히 이해하기 위해서는 메커니즘에 대한 추가 연구와 탐색이 필요하다.

Objectives: This study aimed to investigate the effects of rhubarb extract on osteoclast differentiation in bone marrow-derived macrophages (BMMs). Osteoclasts are vital for bone resorption and remodeling. Osteoclast dysregulation can contribute to various bone-related disorders that directly affect oral health. Rhubarb, a medicinal plant with anti-inflammatory properties, has been shown to modulate bone metabolism. Methods: BMMs were isolated from the femurs and tibias of 5-week-old C57BL/6 mice and cultured in the presence of mouse macrophage colony-stimulating factor (M-CSF) for 3 days. Subsequently, BMMs were treated with M-CSF and receptor activator of nuclear factor-κB ligand (RANKL) to induce osteoclast differentiation. Results: Rhubarb extract effectively suppressed osteoclast differentiation in BMMs. Furthermore, rhubarb extract inhibited the mRNA expression of tartrate-resistant acid phosphatase (TRAP) and cathepsin K (CTSK), which are essential for osteoclastogenesis. Moreover, it inhibited the RANKL-induced expression of nuclear factor of activated T cell c1 (NFATc1), a crucial transcription factor in osteoclast differentiation. Conclusions: These results suggest that rhubarb extract promotes oral health by inhibiting osteoclastogenesis in BMMs. Thus, rhubarb extract shows promise as a therapeutic agent for bone-related disorders that directly affect oral health, particularly those associated with abnormal osteoclast activity. Further research and exploration of the underlying mechanisms are warranted to fully understand their potential clinical applications.

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참고문헌

  1. Anil S, Preethanath RS, AlMoharib HS, Kamath KP, Anand PS. Impact of osteoporosis and its treatment on oral health. Am J Med Sci 2013;346(5):396-401. https://doi.org/10.1097/MAJ.0b013e31828983da 
  2. Wu Y, Yang Y, Wang L, Chen Y, Han X, Sun L, et al. Effect of bifidobacterium on osteoclasts: Tnf-alpha/nf-kappab inflammatory signal pathway-mediated mechanism. Front Endocrinol (Lausanne) 2023;14:1109296. https://doi.org/10.3389/fendo.2023.1109296 
  3. Ishtiaq S, Fogelman I, Hampson G. Treatment of post-menopausal osteoporosis: beyond bisphosphonates. J Endocrinol Invest 2015;38(1):13-29. https://doi.org/10.1007/s40618-014-0152-z 
  4. Gao H, He C, Hua R, Liang C, WangB, Du Y, et al. Underlying beneficial effects of rhubarb on constipation-induced inflammation, disorder of gut microbiome and metabolism. Front Pharmacol 2022;13:1048134. https://doi.org/10.3389/fphar.2022.1048134 
  5. Kawamoto D, Ando-Suguimoto ES, Bueno-Silva B, DiRienzo JM, Mayer MP. Alteration of homeostasis in pre-osteoclasts induced by aggregatibacter actinomycetemcomitans cdt. Front Cell Infect Microbiol 2016;6:33. https://doi.org/10.3389/fcimb.2016.00033 
  6. Cardoneanu A, RezusC, TambaBI, Rezus E.Bone cells metabolic changes induced by ageing. Subcell Biochem 2023;103:13-29. https://doi.org/10.1007/978-3-031-26576-1_2 
  7. Kamboj MK. Metabolic bone disease in adolescents: recognition, evaluation, treatment, and prevention. Adolesc Med State Art Rev 2007;18(1):24-46, viii.  https://doi.org/10.1542/9781581104066-metabolic
  8. Amato M, Santonocito S, Viglianisi G, Tatullo M, Isola G. Impact of oral mesenchymal stem cells applications as a promising therapeutic target in the therapy of periodontal disease. Int J Mol Sci 2022;23(21):13419. https://doi.org/10.3390/ijms232113419 
  9. Ge Y, Sun M, Salome-Abarca LF, Wang M, Choi YH. Investigation of species and environmental effects on rhubarb roots metabolome using (1)h nmr combined with high performance thin layer chromatography. Metabolomics 2018;14(10):137. https://doi.org/10.1007/s11306-018-1421-1 
  10. Semwal RB, Semwal DK, Combrinck S, Viljoen A. Emodin-a natural anthraquinone derivative with diverse pharmacological activities. Phytochemistry 2021;190:112854. https://doi.org/10.1016/j.phytochem.2021.112854 
  11. Kim K, Kim TH, Ihn HJ, Kim JE, Choi JY, Shin HI, et al. Inhibitory effect of purpurogallin on osteoclast differentiation in vitro through the downregulation of c-Fos and NFATc1. Int J Mol Sci 2018;19(2):601. https://doi.org/10.3390/ijms19020601 
  12. ShibataK, Yoshimura Y, KikuiriT, Hasegawa T, Taniguchi Y, Deyama Y, et al. Effect of the release from mechanical stress on osteoclastogenesis in raw 264.7 cells. Int J Mol Med 2011;28(1):73-9. https://doi.org/10.3892/ijmm.2011.675 
  13. Caballe-Serrano J, Cvikl B, Bosshardt DD, Buser D, Lussi A, Gruber R. Saliva suppresses osteoclastogenesis in murine bone marrow cultures. J Dent Res 2015;94(1):192-200. https://doi.org/10.1177/0022034514553977 
  14. Chaweewannakorn W, Ariyoshi W, Okinaga T, Fujita Y, Maki K, Nishihara T. Ameloblastin attenuates rankl-mediated osteoclastogenesis by suppressing activation of nuclear factor of activated t-cell cytoplasmic 1 (nfatc1). JCell Physiol 2019;234(2):1745-57. https://doi.org/10.1002/jcp.27045 
  15. Matsubara T, Kokabu S, Nakatomi C, Kinbara M, Maeda T, Yoshizawa M, et al. The actin-binding protein ppp1r18 regulates maturation, actin organization, and bone resorption activity of osteoclasts. Mol Cell Biol 2018;38(4):e00425-17. https://doi.org/10.1128/MCB.00425-17 
  16. Matsunaga A, Takami M, Irie T, Mishima K, Inagaki K, Kamijo R. Microscopic study on resorption of beta-tricalcium phosphate materials by osteoclasts. Cytotechnology 2015;67(4):727-32. https://doi.org/10.1007/s10616-015-9854-0