Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Effect of Paeoniae Radix Alba against UVB Photodamage

UVB 광손상에 대한 백작약의 보호 효과

  • Sook Jahr Park (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Jong Rok Lee (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 박숙자 (대구한의대학교 제약공학과) ;
  • 이종록 (대구한의대학교 제약공학과)
  • Received : 2023.08.16
  • Accepted : 2023.08.22
  • Published : 2023.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : UVB damages skin health by causing skin redness and intense inflammation, sunburn, and skin cancer. Paeoniae Radix Alba has been used to relieve gynecological symptoms, muscle spasms, and skin ailments. This study was conducted to confirm whether it has a protective effect against UVB photodamage. Methods : Ethanol extract of Paeoniae Radix Alba (PRA) was prepared by extracting 100 g Paeoniae Radix Alba in 1 L of ethanol for 48 h. Apoptosis was monitored by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and expression levels of apoptosis indicator proteins, and tyrosinase activity was measured with a colorimetric commercial kit. Results : In human keratinocyte HaCaT cells, PRA reduced UVB-induced cell death through apoptosis by inhibiting PARP cleavage and caspase-3 and -9. UVB-induced increase in cellular reactive oxygen species (ROS) was suppressed by PRA pretreatment. PRA also showed dose-dependent ABTS and DPPH radical scavenging activities. Furthermore, the inhibitory effect of tyrosinase activity by PRA was confirmed. Conclusion : These results demonstrated the protective role of PRA in UVB photodamage of human keratinocytes, mainly due to its antioxidant and antiapoptotic properties. We also suggest that PRA can be considered as an effective natural agent to prevent skin photodamage.

Keywords

Acknowledgement

이 논문은 2023년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2018R1A5A2025272).

References

  1. Kim DG, Kim MB, Kim H, Park JH, Lim JP, Hong SH. Herb Medicinal Pharmacognosy. Seoul : Shinil. 2005:383-4.
  2. Oriental Pharmacology Committee. Oriental Pharmacology. Seoul : Shinil. 2006:133-4.
  3. Professors of Herbal Formula in Korea. Herbal Formula. Seoul : Younglim. 2000:581.
  4. Kook YB, Lee JC, Kim HS. Effects of Jakyakgamchotang Extract on the Trachea Smooth Muscle. Herb Formula Sci. 2002;10(2):143-58.
  5. Gushi A, Koriyama C, Yoshinaga R, Yano H, Tsumagari J, Tahara E. Efficacy of Kampo Medicine for Acne of the Face with Nonreactive Standard Dermatological Therapy. Kampo Med. 2016;67(2):123-30. https://doi.org/10.3937/kampomed.67.123
  6. Lee JY, Bae HJ, Park TS, Kim TW, Moon DH, Kwon OJ et al. Anti-oxidant and Anti-microbial Activities of Seungmakalgeuntang. J Appl Biol Chem. 2010;53(1):13-20. https://doi.org/10.3839/jabc.2010.003
  7. Keum JH, Seo YS, Kang OH, Choi JG, Kwon DY. Anti-inflammatory effect of Seungmagalgeun-tang extract in human mast cells. Kor J Herbol. 2013;28(5):7-11. https://doi.org/10.6116/kjh.2013.28.5.7
  8. Bae JH. Effect of Extracts from Paeonia japonica on the Growth of Food-borne Pathogens. J East Asian Soc Dietary Life. 2011;21(2):272-6.
  9. Jeong IY, Lee JS, Oh H, Jung U, Park HR, Jo SK. Inhibitory Effect of Hot-Water Extract of Paeonia japonica on Oxidative Stress and Identification of Its Active Components. J Korean Soc Food Sci Nutr. 2003;32(5):739-44. https://doi.org/10.3746/jkfn.2003.32.5.739
  10. Kim DY, Kim MK. Anti-inflammatory Effect of Paeonia japonica Extracts in HaCaT cells. J Invest Cosmetol. 2019;15(3):247-54.
  11. Kim JH, Kimm EJ. Evaluation of Anti-oxidative, Anti-thrombin, Anti-invasive and Pro-apoptotic Activities of Paeonia japonica. Korean J Plant Res. 2018;31(1):16-23.
  12. Kim MY, Yoon BY, Ko KS. Study on Cosmeceutical Activities of Paeonia japonica Ethanol Extracts. J Kor Soc Cosmetol. 2016;22(4):818-23.
  13. Lee JC, Park SY, Choi JH, Kim JH. Effects of Paeoniae radix alba(PRA) on Skin whitening and Elasticity using Melanoma cells. JKOOD. 2012;25(1):1-11.
  14. Amaro-Ortiz A, Yan B, D'Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014;19(5):6202-19. https://doi.org/10.3390/molecules19056202
  15. Murayama N, Murayama K. Data on substantial gravity of carbon dioxide due to pressured metered-dose inhaler steroid treatments for the 2006 year in Japan. Data Brief. 2018;20:1580-6. https://doi.org/10.1016/j.dib.2018.08.070
  16. Bessou S, Surleve-Bazeille JE, Sorbier E, Taieb A. Ex vivo reconstruction of the epidermis with melanocytes and the influence of UVB. Pigment Cell Res. 1995;8(5):241-9. https://doi.org/10.1111/j.1600-0749.1995.tb00670.x
  17. Yardman-Frank JM, Fisher DE. Skin pigmentation and its control: From ultraviolet radiation to stem cells. Exp Dermatol. 2021;30(4):560-71. https://doi.org/10.1111/exd.14260
  18. Catani MV, Rossi A, Costanzo A, Sabatini S, Levrero M, Melino G, Avigliano L. Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage. Biochem J. 2001;356(Pt 1):77-85. https://doi.org/10.1042/bj3560077
  19. Yoon SD, Baek WK, Kim SP, Lee KS, Cho JW. Induction of Apoptosis and Autophagy in UVB-Treated HaCaT Cells. Korean J Dermatol. 2013;51(8):600-7.
  20. Tyrrell RM. Ultraviolet radiation and free radical damage to skin. Biochem Soc Symp. 1995;61:47-53. https://doi.org/10.1042/bss0610047
  21. Eckhart L, Zeeuwen PLJM. The skin barrier: Epidermis vs environment. Exp Dermatol. 2018;27(8):805-6. https://doi.org/10.1111/exd.13731
  22. Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol. 2007;35:495-516. https://doi.org/10.1080/01926230701320337
  23. Paz ML, Gonzalez Maglio DH, Weill FS, Bustamante J, Leoni J. Mitochondrial dysfunction and cellular stress progression after ultraviolet B irradiation in human keratinocytes. Photodermatol Photoimmunol Photomed. 2008;24(3):115-22. https://doi.org/10.1111/j.1600-0781.2008.00348.x
  24. Samraj AK, Sohn D, Schulze-Osthoff K, Schmitz I. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membrane depolarization. Mol Biol Cell. 2007;18:84-93. https://doi.org/10.1091/mbc.e06-04-0263
  25. Wang CB, Huang MQ, Tao GL, Yu GY, Han ZW, Yang ZH, Wang YJ. Polypeptide from Chlamys farreri protects HaCaT cells from UVB-induced apoptosis. Chem Biol Interact. 2004;147(2):119-27. https://doi.org/10.1016/j.cbi.2003.11.002
  26. Masaki H, Atsumi T, Sakurai H. Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem Biophys Res Commun. 1995;206(2):474-9. https://doi.org/10.1006/bbrc.1995.1067
  27. Dwivedi A, Mujtaba SF, Kushwaha HN, Ali D, Yadav N, Singh SK et al. Photosensitizing mechanism and identification of levofloxacin photoproducts at ambient UV radiation. Photochem Photobiol. 2012;88(2):344-55. https://doi.org/10.1111/j.1751-1097.2011.01068.x
  28. Shalaby EA, Shanab MM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo-Marine Sciences. 2013;42(5):556-64.
  29. Rescigno A, Sollai F, Pisu B, Rinaldi A, Sanjust E. Tyrosinase inhibition: general and applied aspects. J Enzyme Inhib Med Chem. 2002;17(4):207-18.  https://doi.org/10.1080/14756360210000010923