The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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A Mutation of a Putative NDP-Sugar Epimerase Gene in Ralstonia pseudosolanacearum Attenuates Exopolysaccharide Production and Bacterial Virulence in Tomato Plant

  • Hyoung Ju Lee (Department of Applied Bioscience, Dong-A University) ;
  • Sang-Moo Lee (Institute of Agricultural Life Sciences, Dong-A University) ;
  • Minseo Choi (Department of Applied Bioscience, Dong-A University) ;
  • Joo Hwan Kwon (Department of Applied Bioscience, Dong-A University) ;
  • Seon-Woo Lee (Department of Applied Bioscience, Dong-A University)
  • 투고 : 2023.06.29
  • 심사 : 2023.08.21
  • 발행 : 2023.10.01
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초록

Ralstonia solanacearum species complex (RSSC) is a soil borne plant pathogen causing bacterial wilt on various important crops, including Solanaceae plants. The bacterial pathogens within the RSSC produce exopolysaccharide (EPS), a highly complicated nitrogencontaining heteropolymeric polysaccharide, as a major virulence factor. However, the biosynthetic pathway of the EPS in the RSSC has not been fully characterized. To identify genes in EPS production beyond the EPS biosynthetic gene operon, we selected the EPS-defective mutants of R. pseudosolanacearum strain SL341 from Tn5-inserted mutant pool. Among several EPSdefective mutants, we identified a mutant, SL341P4, with a Tn5-insertion in a gene encoding a putative NDP-sugar epimerase, a putative membrane protein with sugar-modifying moiety, in a reverse orientation to EPS biosynthesis gene cluster. This protein showed similar to other NDP-sugar epimerases involved in EPS biosynthesis in many phytopathogens. Mutation of the NDP-sugar epimerase gene reduced EPS production and biofilm formation in R. pseudosolanacearum. Additionally, the SL341P4 mutant exhibited reduced disease severity and incidence of bacterial wilt in tomato plants compared to the wild-type SL341 without alteration of bacterial multiplication. These results indicate that the NDP-sugar epimerase gene is required for EPS production and bacterial virulence in R. pseudosolanacearum.

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과제정보

This work was supported by National Research Foundation of Korea (NRF) grants to S.-W. L. (No. 2020R1A2C3005453 and 2020R1A6A1A03047729) and Green Fusion Technology Program funded by the Korea government (MSIT, MOE, ME), Republic of Korea.

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