The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Molecular Marker Development for the Rapid Differentiation of Black Rot Causing Xanthomonas campestris pv. campestris Race 7

  • Yeo-Hyeon Kim (Department of Horticulture, Sunchon National University) ;
  • Sopheap Mao (Department of Horticulture, Sunchon National University) ;
  • Nihar Sahu (Department of Horticulture, Sunchon National University) ;
  • Uzzal Somaddar (Department of Horticulture, Sunchon National University) ;
  • Hoy-Taek Kim (Department of Horticulture, Sunchon National University) ;
  • Masao Watanabe (Graduate School of Life Science, Tohoku University) ;
  • Jong-In Park (Department of Horticulture, Sunchon National University)
  • 투고 : 2023.07.20
  • 심사 : 2023.09.12
  • 발행 : 2023.10.01
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초록

Xanthomonas campestris pv. campestris (Xcc) is a plant pathogen of Brassica crops that causes black rot disease throughout the world. At present, 11 physiological races of Xcc (races 1-11) have been reported. The conventional method of using differential cultivars for Xcc race detection is not accurate and it is laborious and time-consuming. Therefore, the development of specific molecular markers has been used as a substitute tool because it offers an accurate and reliable result, particularly a quick diagnosis of Xcc races. Previously, our laboratory has successfully developed race-specific molecular markers for Xcc races 1-6. In this study, specific molecular markers to identify Xcc race 7 have been developed. In the course of study, whole genome sequences of several Xcc races, X. campestris pv. incanae, X. campestris pv. raphani, and X. campestris pv. vesicatoria were aligned to identify variable regions like sequence-characterized amplified regions and insertions and deletions specific to race 7. Primer pairs were designed targeting these regions and validated against 22 samples. The polymerase chain reaction analysis revealed that three primer pairs specifically amplified the DNA fragment corresponding to race 7. The obtained finding clearly demonstrates the efficiency of the newly developed markers in accurately detecting Xcc race 7 among the other races. These results indicated that the newly developed marker can successfully and rapidly detect Xcc race 7 from other races. This study represents the first report on the successful development of specific molecular markers for Xcc race 7.

키워드

과제정보

This work was supported by grants from Ministry of Agriculture, Food and Rural Affairs (MAFRA) (322059-03-2-HD050). We would thank Dr. Pilar Soengas, Department of Plant Genetics, Spain for providing Xcc race 8. We also thank Dr. Joana G. Vicente, University of Warwick, UK for providing Xcc races (races 1-7) and Xc pathovars. We thank the Korean Agriculture Culture Collection (KACC), Korea for providing Xcc species and ICMP collection, New Zealand for providing isolates of plant pathogenic bacteria.

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