BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Photobiomodulation therapy activates YAP and triggers proliferation and dedifferentiation of Müller glia in mammalian retina

  • Seo-Yeon Kim (Myung-Gok Eye Research Institute, Konyang University) ;
  • Myung-Jun Song (Myung-Gok Eye Research Institute, Konyang University) ;
  • In-Beom Kim (Department of Anatomy, College of Medicine, The Catholic University of Korea) ;
  • Tae Kwan Park (Department of Ophthalmology, Soonchunhyang University Hospital Bucheon, Soonchunhyang University) ;
  • Jungmook Lyu (Myung-Gok Eye Research Institute, Konyang University)
  • 투고 : 2023.04.15
  • 심사 : 2023.05.28
  • 발행 : 2023.09.30
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초록

Photobiomodulation therapy has been proposed as a promising therapeutic approach for retinal degenerative diseases. However, its effect on the regenerative capacity in mammalian retina and its intracellular signalling mechanisms remain unknown. Here, we show that photobiomodulation with 670 nm light stimulates Müller glia cell cycle re-entry and dedifferentiation into a progenitor-like state in both the uninjured and injured retina. We also find that 670 nm light treatment inhibits the Hippo pathway, which is activated in Müller glia following NaIO3-induced retinal injury. YAP, a major downstream effector of the Hippo signalling pathway was translocated into the nucleus of Müller glia along with YAP dephosphorylation in retina treated with 670 nm light. Deficiency of YAP attenuated Müller glia cell cycle re-entry and dedifferentiation. Our data reveal that the Hippo-YAP signalling pathway is associated with the photostimulatory effect on regenerative response in mammalian retina, and suggest a potential therapeutic strategy for retinal degenerative diseases.

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과제정보

This research was supported by the Konyang University Research Fund (the second half of 2021) and KHIDI grant (HI 22C0733).

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