식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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메타전사체 분석을 이용한 국내 대추나무의 바이러스 감염실태

Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea

  • 이홍규 (경북대학교 응용생명과학부) ;
  • 한승주 (경북대학교 응용생명과학부) ;
  • 박상민 (경북대학교 응용생명과학부) ;
  • 김민석 (경북대학교 응용생명과학부) ;
  • 민진경 (경북대학교 응용생물학과) ;
  • 김학주 (경북대학교 응용생명과학부) ;
  • 강동현 (경북대학교 응용생명과학부) ;
  • 김민희 (경북대학교 응용생명과학부) ;
  • 정원영 (경북대학교 응용생명과학부) ;
  • 백승빈 (경북대학교 응용생물학과) ;
  • 양민주 (경북대학교 응용생물학과) ;
  • 임태건 (경북대학교 응용생물학과) ;
  • 안찬훈 (국립산림과학원 임목자원연구과) ;
  • 김태동 (국립산림과학원 산림특용자원연구과) ;
  • 박충열 (국립백두대간수목원 야생식물종자연구실) ;
  • 문제선 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이수헌 (경북대학교 식물의학과)
  • Hong-Kyu Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Seongju Han (School of Applied Biosciences, Kyungpook National University) ;
  • Sangmin Bak (School of Applied Biosciences, Kyungpook National University) ;
  • Minseok Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Jean Geung Min (Department of Applied Biology, Kyungpook National University) ;
  • Hak ju Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Dong Hyun Kang (School of Applied Biosciences, Kyungpook National University) ;
  • Minhui Kim (School of Applied Biosciences, Kyungpook National University) ;
  • Wonyoung Jeong (School of Applied Biosciences, Kyungpook National University) ;
  • Seungbin Baek (Department of Applied Biology, Kyungpook National University) ;
  • Minjoo Yang (Department of Applied Biology, Kyungpook National University) ;
  • Taegun Lim (Department of Applied Biology, Kyungpook National University) ;
  • Chanhoon An (Division of Forest Tree Improvement and Biotechnology, National Institute of Forest Science) ;
  • Tae-Dong Kim (Division of Special Forest Resources, National Institute of Forest Science) ;
  • Chung Youl Park (Division of Wild Plant Seed Research, Baekdudaegan National Arboretum) ;
  • Jae Sun Moon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Su-Heon Lee (Department of Plant Medicine, Kyungpook National University)
  • 투고 : 2023.06.09
  • 심사 : 2023.07.05
  • 발행 : 2023.09.30
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초록

본 연구는 국내 대추나무 바이러스 감염실태를 구명하기 위하여 시험포장 및 농가포장에서 다양한 이상증상을 나타내는 시료 61점을 채집하였다. 이후 채집한 시료는 메타전사체 분석, reverse transcription polymerase chain reac tion 진단, 염기서열 분석에 사용하였다. 그 결과 국내 대추나무에서는 2종의 DNA 바이러스, jujube associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV)와 1종의 RNA 바이러스, jujube yellow mottle-associated virus (JYMaV)를 동정하였다. 채집된 모든 시료는 3종 바이러스에 단독 또는 복합감염되어 있음을 확인하였다. 바이러스별 검출은 JuBV 100%, JYMaV 90.2%, JuMaV 8.2%로 나타났다. 3종 바이러스의 감염조합은 JuBV 단독감염 9.8%, JuBV+JYMaV 2종 복합감염 82.0%, JuBV+JYMaV+JuMaV 3종 복합감염 8.2%로 나타났다. 국내 대추나무에서 검출된 3종 바이러스의 염기서열 분석결과 중국에서 보고된 각각의 바이러스 분리주와 매우 높은 상동성을 나타내었다. 본 논문은 대추나무 바이러스 무병묘 생산을 위한 기초자료로 활용될 수 있을 것으로 기대되며, 국내 대추나무에서 바이러스 감염실태와 JuBV와 JuMaV에 대한 첫 보고이다.

This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.

키워드

과제정보

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. RS-2023-00259922)" Rural Development Administration, Republic of Korea and "Development of virus-free plant production technology based on BT (Project No. FG0700-2018-02-2021)" National Institute of Forest Science, Republic of Korea. We would like to thank Enago (www.enago.co.kr) for editing and reviewing this manuscript for English language.

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