Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Genome-Based Insights into the Thermotolerant Adaptations of Neobacillus endophyticus BRMEA1T

  • Lingmin Jiang (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ho Le Han (The University of Danang, University of Science and Technology) ;
  • Yuxin Peng (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Doeun Jeon (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Donghyun Cho (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Cha Young Kim (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jiyoung Lee (Biological Resource Center, Korea Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2023.06.22
  • Accepted : 2023.08.21
  • Published : 2023.09.30
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Abstract

The bacterium Neobacillus endophyticus BRMEA1T, isolated from the medicinal plant Selaginella involvens, known as its thermotolerant can grow at 50℃. To explore the genetic basis for its heat tolerance response and its potential for producing valuable natural compounds, the genomes of two thermotolerant and four mesophilic strains in the genus Neobacillus were analyzed using a bioinformatic software platform. The whole genome was annotated using RAST SEED and OrthVenn2, with a focus on identifying potential heattolerance-related genes. N. endophyticus BRMEA1T was found to possess more stress response genes compared to other mesophilic members of the genus, and it was the only strain that had genes for the synthesis of osmoregulated periplasmic glucans. This study sheds light on the potential value of N. endophyticus BRMEA1T, as it reveals the mechanism of heat resistance and the application of secondary metabolites produced by this bacterium through whole-genome sequencing and comparative analysis.

Keywords

Acknowledgement

This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Agricultural Machinery/Equipment Localization Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321057051HD020), and by the KRIBB research initiative program (KGM5282331).

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