Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of GA3 Treatment on Seed Germination, Seedling Growth and Useful Component Content of Angelica acutiloba (Siebold & Zucc.) Kitagawa

GA3 처리 왜당귀의 종자 발아, 유묘 생육 및 유용성분 특성 분석

  • Dae Hui Jeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Hong Woo Park (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Young Ki Kim (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Hae Yun Kwon (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 정대희 (국립산림과학원 산림약용자원연구소) ;
  • 박홍우 (국립산림과학원 산림약용자원연구소) ;
  • 김영기 (국립산림과학원 산림약용자원연구소) ;
  • 권해연 (국립산림과학원 산림약용자원연구소)
  • Received : 2023.07.17
  • Accepted : 2023.09.05
  • Published : 2023.10.01
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Abstract

Germination, growth characteristics, and content of useful components were analyzed through GA3 treatment of Angelica acutiloba (Siebold & Zucc.) Kitagawa seeds, to provide basic data for cultivation technology. The moisture absorption rate of the seeds was 62.07% after 2 hours of soaking, and the highest value was 122.15% after 48 hours. The appropriate seed germination temperature for the species ranged from 10℃ to 25℃. It was confirmed that the growth of seedlings increased as the GA3 concentration increased, but there was no significant difference in the content of useful components according to the GA3 treatment.

왜당귀[Angelica acutiloba (Siebold & Zucc.) Kitagawa]를 대상으로 종자 발아 및 유묘 생육, 유용성분 함량과 관련된 GA3 처리 효과를 분석하여 왜당귀 재배시 기초자료를 제공하고자 하였다. 왜당귀 종자의 수분흡수율은 침지 2시간이 경과한 시점에서 62.07%를 나타냈고, 48시간이 경과한 시점에서 122.15%로 가장 높은 값을 나타냈다. 왜당귀 종자의 적정 발아온도는 10℃에서 25℃로 확인되었으며, 5℃에서는 발아가 진행되지 않았고, 30℃에서는 GA3 농도가 증가함에 따라 발아율이 유의하게 증가하였다. 또한 유묘 생육은 GA3 처리농도가 높아질수록 양호하였으나 유용성분 함량의 경우 실험구에 따른 유의성이 확인되지 않았다. 이러한 결과는 약용 및 식용으로 활용성이 높은 왜당귀에 대한 최적 재배와 생산기술 정립에 기초자료로 활용할 수 있을 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 국립산림과학원 연구개발사업(과제번호: FP0802-2023-01-2023)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

References

  1. Achard, P. and P. Genschik. 2009. Releasing the brakes of plant growth: how GAs shutdown DELLA proteins. J. Exp. Bot. 60(4):1085-1092. https://doi.org/10.1093/jxb/ern301
  2. Baskin, C.C. and J.M. Baskin. 2003. When breaking seed dormancy is a problem try a move-along experiment. Nat Plants 4:17-21. https://doi.org/10.3368/npj.4.1.17
  3. Baskin, J.M. and C.C. Baskin. 2004. A classification system for seed dormancy. Seed Sci. Res. 14:1-16. https://doi.org/10.1079/SSR2003150
  4. Chen, S.S.C. and J.L.L. Chang. 1972. Does gibberellic acid stimulate seed germination via amylase synthesis?. Plant Physiol. 49:441-442. https://doi.org/10.1104/pp.49.3.441
  5. Cho, N.J., W.H. Lee, K.K. Kim and H.S. Han. 2017. Investigation of the antioxidant effect of Angelicae Radix from Korea, China and Japan. J. Physiol & Pathol Korean Med. 31(3):182-187 (in Korean). https://doi.org/10.15188/kjopp.2017.06.31.3.182
  6. Choi, J.E., M.R. Jeong, K.Y. Lee, B.C. Moon and Y.M. Kang. 2015. Rapid in vitro propagation and accumulized of Angelica acutiloba Kitag. using the selected seeds. Korean Herb. Med. Inf. 3(2):33-41 (in Korean).
  7. Choi, J.W., J.H. Lee, W.B. Kim, C.K. Kim, H.K. Jung, Y.P. Hong and J.G. Kim. 2017. Changes in the quality and physiological activity of Angelica acutiloba leaves in various packaging materials during storage. Korean J. Plant Res. 30(1):29-37 (in Korean). https://doi.org/10.7732/kjpr.2016.30.1.029
  8. Daviere, J.M. and P. Achard. 2013. Gibberellin signaling in plants. Development 140:1147-1151. https://doi.org/10.1242/dev.087650
  9. Dhoran, V.S. and S.P. Gudadhe. 2012. Effect of plant growth regulators on seed germination and seedling vigour in Asparagus sprengeri Regelin. I. Res. J. Biological Sci. 1(7):6-10.
  10. Eom, H.J., Y.Y. Jeong, N.R. Kwon, K.H. Kim, E. Yeon, H.S. Yoon, Y.J. Ryu and I.J. Kim. 2021. Nutritional components and physiological activity of 4 wild vegetables (Salvia plebeia R. Br, Angelica acutiloba, Gynura procumbens and Saururus chinensis Baill) cultivated in Chungbuk province. Korean J. Food Nutr. 34(4):398-406 (in Korean).
  11. Finch-Savage, W.E. and G. Leubner-Metzger. 2006. Seed dormancy and the control of germination. New Phytol.171:501-523. https://doi.org/10.1111/j.1469-8137.2006.01787.x
  12. Fu, X. and N.P. Harberd. 2003. Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421:740-743. https://doi.org/10.1038/nature01387
  13. Geshnizjani, N., F. Ghaderi-far, L.A. Willems, H.W.M. Hillhorst and W. Ligterink. 2018. Characterization of and genetic variation for tomato seed thermo-inhibition and thermos-dormancy. BMC Plant Biol. 18:229.
  14. Han, H.S. 2013. Anti-inflammatory effect of Angelicae acutilobae Radix water extract on LPS-stimulated mouse macrophages. Kor. J. Herbology 28(6):129-133 (in Korean). https://doi.org/10.6116/kjh.2013.28.6.129
  15. Han, S.B., Y.H. Kim, C.W. Lee, S.W. Park, H.Y. Lee, K.S. Ahn, I.H. Kim and H.M. Kim. 1998. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Int. Immunopharmacol. 40(1):39-48. https://doi.org/10.1016/S0162-3109(98)00026-5
  16. Han, S.H., J.J. Ku, D.H. Kim and H.I. Lim. 2019. Effects of gibberellin treatment on the dormancy-breaking and germination promotion of Carpinus laxiflora seeds stored for 16 years. J. Korean Soc. For. Sci. 108(4):531-539 (in Korean).
  17. Hifny, H.A., S.M. Khalifa, A.E. Hamdy and A.N. Abd ElWahed. 2017. Effect of GA3 and NAA on growth, yield and fruit quality of washington navel orange. Egypt. J. Hort. 44(1):33-43. https://doi.org/10.21608/ejoh.2017.950.1003
  18. Je, B.I., J.S. Jeon, J.S. Kang and Y.H. Choi. 2023. Gibberellic acid and cold stratification promotes seed germination and seedling growth in Kadsura coccinea. J. Environ. Sci. Int. 32(1):77-88 (in Korean). https://doi.org/10.5322/JESI.2023.32.1.77
  19. Jeong, H.M., E.J. Kim and D.G. Choi. 2018. Effects of GA3 and streptomycin on seedless fruit formation in blackberry. Hortic. Sci. Technol. 36(5):658-665 (in Korean). https://doi.org/10.12972/kjhst.20180066
  20. Kang, M.S, H.Y. Ha and H.T. Kim. 2015. An experimental study on the effect of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 28(1):32-40 (in Korean). https://doi.org/10.6114/jkood.2015.28.1.032
  21. Kang, S.A., K.H. Jang, J.E. Lee, D.K. Ahn and S.K. Park. 2003. Differences of hematopoietic effects of Angelica gigas, A. sinensis and A. acutiloba extract on cyclophosphamide-induced anemic rats. Korean J. Food Sci. Technol. 35(6):1204-1208 (in Korean).
  22. Kim, A.R., J.J. Lee and M.Y. Lee. 2009. Antioxidative effect of Angelica acutiloba Kitagawa ethanol extract. J. Life Sci. 19(1):117-122 (in Korean). https://doi.org/10.5352/JLS.2009.19.1.117
  23. Kim, G.M., J.M. Chung, J.Y. Jung, H.J. Choi and S.Y. Lee. 2022. Germination characteristics in seeds of Broussonetia kazinoki Siebold ex Siebold & Zucc (Moraceae) native to East Asia. Korean J. Plant Res. 35(1):36-43 (in Korean).
  24. Kim, S.A., H.K. Oh, J.Y. Kim, J.W. Hong and S.I. Cho. 2011. Areview of pharmacological effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their bioactive compounds. J. Korean Oriental Med. 32(4):1-24 (in Korean).
  25. Kim, Y.H. and I.J. Lee. 2013. Influence of plant growth regulator application on seed germination of dandelion (Taraxacum officinale). Weed Turf. Sci. 2(2):152-158 (in Korean). https://doi.org/10.5660/WTS.2013.2.2.152
  26. Kim, Y.H., A.L. Khan, M. Hamayun, J.T. Kim, J.H. Lee, I.C. Hwang, C.S. Yoon and I.J. Lee. 2009. Effects of prohexadione calcium on growth and gibberellins contents of Chrysanthemum morifolium R. cv. Monalisa white. Sci. Hortic. 123:423-427. https://doi.org/10.1016/j.scienta.2009.09.022
  27. Kwon, H.H., M. Gil, Y.H. Kwon, H.J. Kwon, S.Y. Kim and Y.H. Rhie. 2020. Seed dormancy of Viola mandshurica and V. albida and their germination characteristics by seed storage. Flower Res. J. 28(3):132-138 (in Korean). https://doi.org/10.11623/frj.2020.28.3.04
  28. Lee, E.S., T.J. An, Y.I. Kim, W.T. Park, Y.G. Kim and J.K. Chang. 2020a. Seed ripening characteristics by inflorescences according to days after flowering in Angelica acutiloba (Siebold & Zucc.) Kitagawa. Korean J. Plant Res. 33(3):86-92 (in Korean).
  29. Lee, G.Y., Y.H. Kim, H.S. Soh and S.D. Lee. 2020b. Effect of benzylamino purine (BA) and gibberellic acid (GA3) on growth and flowering in Schlumbergera truncata. Hortic. Sci. Technol. 38(4):419-428 (in Korean). https://doi.org/10.7235/HORT.20200040
  30. Lee, H., K.U. Kim, T.K. Son, J.E. Lee and S.C. Lee. 2002. Effect of application of plant growth regulator on growth characteristics in Bupleurum falcatum L. Korean J. Medcinal Crop Sci. 10(5):344-352 (in Korean).
  31. Lee, H.S. and J.H. Kim. 2021. Analysis of food consumption behavior due to COVID-19: focusing on MZ generation. J. Digit. Converg. 19(3):47-54 (in Korean).
  32. Lee, J.H., J.W. Kwon and S.Y. Lee. 2001. Effect of GA3 on germination of Perilla frutescens var. acuta Kudo under high temperature. Korean J. Medicinal Crop Sci. 9(3):198-204 (in Korean).
  33. Lee, S.G. 2008. Comparison of activity of Angelica gigas and Angelica acutiloba from Kangwon. Korean J. Orient. Physiol. Pathol. 22(5):1158-1162 (in Korean).
  34. Lee, S.G., H.Y. Kim and J.J. Ku. 2014. Effects of seed storage methods and GA3 application on seed germination and seedling growth of Solanum lyratum Thunb. Korean J. Plant Res. 27(4):365-370 (in Korean). https://doi.org/10.7732/kjpr.2014.27.4.365
  35. Lee, S.G., W.W. Cho, S.H. Lee, K.W. Park, K. Choi and H.D. Kang. 2012. Effects of plant growth regulators on In vitro germination and organ formation of wild Angelica gigas N. Korean Plant Res. 25(4):456-464 (in Korean). https://doi.org/10.7732/kjpr.2012.25.4.456
  36. Lee, T.B. 2003. Coloured Flora of Korea. Vol. 1. Hayangmunsa, Seoul, Korea. pp. 838-844.
  37. Ministry of Agriculture, Food and Rural Affairs (MAFRA). 2022. 2021 An actual output of crop for a special purpose. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea. pp. 80-88.
  38. Moon, J.O., K.W. Park and M.H. Kang. 2003. Effects of treatment of light, temperature and priming on germination of Angelica acutiloba kitagawa seeds. Hortic. Sci. Technol 21(4):434-439 (in Korean).
  39. Ozkan, Y., M. Ucar, K. Yildiz and B. Ozturk. 2016. Pre-harvest gibberellic acid (GA3) treatments play an important role on bioactive compounds and fruit quality of sweet cherry cultivars. Sci. Hortic. 211:358-362. https://doi.org/10.1016/j.scienta.2016.09.019
  40. Park, H.B., C.H. Ko, S.Y. Kim, K.C. Lee, J.H. Kim and J.M. Chung. 2019. Dormancy type and germination characteristics of seeds of Lonicera chrytsantha Turcz. Ex Ledeb (Caprifoliaceae). Korean J. Plant Res. 32(5):457-462 (in Korean).
  41. Park, M.A., M.j. Sim and Y.C. Kim. 2017. Anti-Photoaging effects of Angelica acutiloba root ethanol extract in human dermal fibroblasts. Toxicol. Res. 33(2):125-134. https://doi.org/10.5487/TR.2017.33.2.125
  42. Paroussi, G., D.G. Voyiatzis, E. Paroussis and P.D. Drogoudi. 2002. Growth, flowering and yield responses to GA3 of strawberry grown under different environmental conditions. Sci. Hortic. 96:103-113. https://doi.org/10.1016/S0304-4238(02)00058-4
  43. Rohamare, Y., T.D. Nikam and K.N. Dhumal. 2013. Effect of foliar application of plant growth regulators on growth, yield and essential oil components of Ajwain (Trachyspermum ammi L.). International J. Seed Spices 3(2):34-41.
  44. Salazar-Cerezo, S., N. Martinez-Montiela, J. Garcia-Sancheza, R. Perez-y-Terronb and R.D. Martinez-Contreras. 2018. Gibberellin biosynthesis and metabolism: a convergent route for plants, fungi and bacteria. Microbiol. Res. 208:85-98. https://doi.org/10.1016/j.micres.2018.01.010
  45. Schwechheimer, C. 2012. Gibberellin signaling in plants-the extended version. Front. Plant Sci. 2:107.
  46. Tomar, S., A.K. Dubey, S.K. Singh and V. Ujjwal. 2016. Effect of different levels of NAA, GA3 and 2,4-D on growth and yield of tomato (Lycopersicon esculentum Mill.). Ann. Hortic. 9(1):97-100. https://doi.org/10.5958/0976-4623.2016.00019.0
  47. Yook, M.J., J.S. Lim, J.H. Chung and D.S. Kim. 2019. Miscanthus seed germination enhanced by gibberellin treatment. Weed Turf. Sci. 8(4):299-307 (in Korean).
  48. Yu, H.S., B.H. Kang, D.J. Im, C.G. Kim, Y.G. Kim, S.T. Lee and Y.H. Chang. 1995. Effects of temperture, light, GA3 and storge method on germination of Angelica gigas Nakai. Korean J. Medicinal Crop Sci. 3(1):30-34 (in Korean).
  49. Zi, J., S. Mafu and R.J. Peters. 2014. To gibberellins and beyond! surveying the evolution of (Di) terpenoid metabolism. Annu. Rev. Plant Biol. 65:259-286. https://doi.org/10.1146/annurev-arplant-050213-035705