Mycobiology

  • 제51권4호
  • /
  • Pages.230-238
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  • 2023
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)

  • Parisa Bahadori Ganjabadi (Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University) ;
  • Mohsen Farzaneh (Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University) ;
  • Mohammad Hossein Mirjalili (Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University)
  • 투고 : 2023.02.06
  • 심사 : 2023.06.09
  • 발행 : 2023.08.31
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초록

Glabridin is a well-known active isoflavone found in the root of licorice (Glycyrrhiza glabra L.) that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytes cultures are the most important alternative methods for plant resources conservation and sustainable production of natural compounds, which has received much attention in recent decades. In the present study, an efficient culture condition was optimized for the biomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticola SBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licorice root extract (as an elicitor) were tested. The results showed that the highest and lowest biomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt (5.85 + 0.11 g/l), respectively. The medium culture PCB was produced the highest level of glabridin (7.26 ± 0.44 mg/l), while the lowest level (4.47 ± 0.02 mg/l) was obtained from the medium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51 mg/l) production were observed from the PCB medium adjusted with pH = 6, while the lowest value of both traits was obtained from the same medium with pH = 7. The highest production of total glabridin (10.85 ± 0.84 mg/l) was also obtained from the culture medium treated with 100 mg/l of the plant root extract. This information can be interestingly used for the commercialization of glabridin production for further industrial applications.

키워드

과제정보

The authors express their appreciation to the Research Council of Shahid Beheshti University, Tehran, Iran for their financial support. We also wish to thank Mr. Ali Esmaeili and Mr. Hamid Ahadi for their kind help in data collection and HPLC analysis, respectively. This work is a part of Parisa Bahadori Ganjabadi M.Sc.'s thesis.

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