한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제40권5호
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  • Pages.965-976
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  • 2023
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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인간유래각질형성세포에서 호장근 추출물이 피부장벽 보호능과 보습능에 미치는 영향

The effects of the Reynoutria japonica on skin-barrier and moisturizing in HaCaT cells

  • Eun Jeong Kang (Department of Pharmacy, Kyungsung University) ;
  • Jia Bak (Department of Pharmaceutical Science and Technology, Kyungsung University) ;
  • Yun-Sik Choi (Department of Pharmacy, Kyungsung University)
  • 투고 : 2023.09.25
  • 심사 : 2023.10.24
  • 발행 : 2023.10.31
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초록

호장은 마디풀과에 속하는 다년초로 우리나라를 포함한 동아시아에서 잘 자생한다. 호장근은 호장의 뿌리로 항염증과 진경제로 사용되어 왔으며 유효성분으로 emodin을 포함하고 있다. 피부의 표피는 자극, 해로운 물질을 차단하고 수분 증발을 방지함으로써 체내를 보호하는 중요한 역할을 한다. 본 연구에서는 호장근과 그 유효성분인 emodin이 피부 장벽과 보습능에 미치는 영향에 대해 평가하고자 하였다. 먼저 호장근은 ABTS+ radicals을 우수하게 제거함으로써 항산화 효능이 뛰어남을 확인하였다. 다음으로, 실시간 중합연쇄효소반응을 통해 각질형성세포의 분화에 중요한 역할을 하는 filaggrin의 유전자 발현을 비교한 결과, 호장근과 emodin에 의해 농도-의존적으로 filaggrin mRNA 발현이 증가하였다. 또한, 호장근과 emodin은 히알루론산 합성에 중요한 역할을 하는 HAS-2 mRNA 발현을 유의하게 증가시키는 것으로 나타났다. 종합적으로, emodin을 유효성분으로 포함하는 호장근은 피부 장벽 강화와 보습능 증강을 위한 기능성 화장품 소재로서 활용될 수 있을 것으로 기대된다.

Reynoutria japonica is a perennate plant belonging to Polygonaceae and grows wild in East Asia containing Korea. Roots of Reynoutria japonica (R. japonica), part of roots of Reynoutria japonica, has been used for anti-inflammation and antispasmodics and contains emodin as active compound. Epidermis of skin is crucial roles to defense our body against stimulants, harmful substance and prevent water loss. In this study, we examined the effect of R. japonica and emodin, its active compound, on skin-barrier and moisturizing on HaCaT cells. First, antioxidant effect of R. japonica was prominent by scavenging ABTS+ radicals. Next, we conducted real time PCR and expression of filaggrin mRNA which is crucial role in differentiation of keratinocyte increased by R. japonica and emodin dose-dependently. In addition, R. japonica and emodin significantly elevated the expression of HAS-2 mRNA which play a role in hyaluronic acid synthesis on HaCaT cells. Taken together, R. japonica containing emodin, as active compound has potential as a cosmetic material for enhancing the function of skin-barrier and moisturizing in epidermis.

키워드

과제정보

본 연구는 재단법인부산테크노파크에서 지원하는 지산학협력 기업R&D 지원사업의 지원을 받아 수행된 연구결과이다.

참고문헌

  1. E. J. Kang, Y. Jang, J. Lee, S. H. Kim, S. Kim, J. Bak, Y. Choi, "The Effects of Chamaecyparis obtusa Oil on Anti-Wrinkle, Skin-Barrier and Moisturizing", Journal of the Korean Applied Science and Technology, Vol.40, No.2 pp. 309-321, (2023).
  2. H. B. Kang, Y. Choi, J. Bak, Y. Jang, "Effect of Lysimachia christinae Hance extract on moisturizing functionand protection of skin barrier", The Korean Society of Culture and Convergence, Vol.42, No.12 pp. 941-958, (2020). https://doi.org/10.33645/cnc.2020.12.42.12.941
  3. M. Kim, "Skin Barrier and Protein", Journal of Skin Barrier Research, Vol.11, No.1 pp. 28-34, (2009).
  4. H. Y. Yu, I. J. Yang, V. R. Lincha, I. S. Park, D. Lee, H. M. Shin, "The Effects of the Fruits of Foeniculum vulgare on Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes", Journal of Life Science, Vol.25, No.8 pp. 880-888, (2015). https://doi.org/10.5352/JLS.2015.25.8.880
  5. Hwang J. 화장품과 피부과학 연구동향. pp. 43-49, Korea Health Industry Development Institute, (2001).
  6. J. S. Oh, H. H. Jang, "Epidermal Differentiation and Skin Barrier", Korean Journal of Aesthetics and Cosmetology, Vol.13, No.6 pp. 713-720, (2015).
  7. M. Hashimoto, K. Maeda, "New Functions of Low-Molecular-Weight Hyaluronic Acid on Epidermis Filaggrin Production and Degradation", Cosmetics, Vol.8, No.4 pp. 118, (2021).
  8. E. Kim, Y. Moon, Y. Jang, "Skin Moisturizing Properties and Anti-Inflammatory effects of extracts from Coptis chinensis in HaCaT cells", Journal of the Korean Applied Science and Technology, Vol.38, No.3 pp. 870-888, (2021).
  9. S. Park, S. Ho, D. Kim, Y. Shin, J. O. Lee, Y. Kim, J. M. Lee, Y Jang, "Effects of PB203 on the Skin Photoaging of Ultraviolet B (UVB)-irradiated Hairless Mice and Human keratinocytes", Journal of Biomedical and Translational Research, Vol.23, No.4 pp. 215-234, (2022).
  10. X. Wang, L. Ye, Q. Lai, S. Wen, Z. Long, X. Qiu, P. M. Elias, B. Yang, M. Man, "Altered Epidermal Permeability Barrier Function in the Uninvolved Skin Supports a Role of Epidermal Dysfunction in the Pathogenesis of Occupational Hand Eczema", Skin Pharmacology and Physiology, Vol.33, No.2 pp.94-101, (2020). https://doi.org/10.1159/000506425
  11. F. Reno, V. Rocchetti, M. Migliario, M. Rizzi, M. Cannas, "Chronic Exposure to Cigarette Smoke Increases Matrix metalloproteinases and Filaggrin mRNA Expression in Oral keratinocytes: role of nicotine stimulation", Journal of Oral Oncology, Vol.47, No.9 pp. 827-830, (2011).
  12. G. M. O'Regan, A. Sandilands, W. H. I. McLean, A. D. Irvine, "Filaggrin in Atopic Dermatitis", Journal of Allergy and Clinical Immunology, Vol.122, pp. 689-693, (2008). https://doi.org/10.1016/j.jaci.2008.08.002
  13. M. H. Kwon, K. Min, Y. Kim, "Inhibitory Effects of Peonia japonica Water Extract on Skin Aging (I) - Focussed on Alleviative Effects of Inflammation and Skin Barrier Damage -". The Korean Society of Environmental Health and Toxicology, Vol.24, No.2 pp. 159-167, (2009).
  14. S. H. Kim, "A Study on the Effects of Skin Barrier Damage on TEWL and the Efficacy of Jojoba oil in Skin Barrier Restitution", Korean Journal of Aesthetics and Cosmetology, Vol.3, No.1 pp. 189-200, (2005).
  15. S. B. Kwon, G. T. Lee, S. J. Choi, N. K. Lee, H. W. Park, K. S. Lee, K. K. Lee, K. J. Ahn, I. S. An, "The Effect of Glycerin, Hyaluronic Acid and Silicone Oil on the Hydration, Moisturization and Transepidermal Water Loss in Human Skin", Korean Journal of Aesthetics and Cosmetology, Vol.11, No.4 pp. 761-768, (2013).
  16. E. H. Choi, N. Y. Yoon, "Pathogenesis of Atopic Dermatitis", Journal of the Korean Medical Association, Vol.57, No.3 pp. 218-225, (2014). https://doi.org/10.5124/jkma.2014.57.3.218
  17. M. J. Kim, B. S. Hwang, Y. Hwang, Y. T. Jeong, D. W. Jeong, Y. T. Oh, "Anti-Inflammatory and Antiatopic Effects of Rorippa cantoniensis (Lour.) Ohwi in RAW 264.7 and HaCaT Cells", Molecules, Vol.28, No.14 pp. 5463, (2023).
  18. R. Wang, S. Yan, X. Ma, J. Zhao, Y. Han, Y. Pan, H. Zhao, "The Pivotal Role of Bifida Ferment Lysate on Reinforcing the Skin Barrier Function and Maintaining Homeostasis of Skin Defenses in vitro", Journal of Cosmetic Dermatology, doi:10.1111/jocd.15831, (2023).
  19. S. Park, I. Jo, S. Park, M. Park, Y. Mun, "Poria cocos Extract from Mushrooms Stimulates Aquaporin-3 via the PI3K/Akt/mTOR Signaling Pathway", Clinical, Cosmetic and Investigational Dermatology, Vol.15, pp. 1919-1931, (2022). https://doi.org/10.2147/CCID.S378545
  20. Y. Yoshino, K. Marunaka, M. Kobayashi, H. Matsunaga, S. Shu, T. Matsunaga, A. Ikari, "Protective Effects of Ethanol Extract of Brazilian Green Propolis and Apigenin against Weak Ultraviolet Ray-B-Induced Barrier Dysfunction via Suppressing Nitric Oxide Production and Mislocalization of Claudin-1 in HaCaT Cells", International Journal of Molecular Sciences, Vol.22, No.19 pp. 10326, (2021).
  21. K. Yang, Y. Sung, H. K. Kim, "Antithrombotic Effect and Antiplatelet Activity of Polygonum cuspidatum Extract", The Korean Society of Food Science and Nutrition, Vol.41, No.2 pp. 168-173, (2012). https://doi.org/10.3746/jkfn.2012.41.2.168
  22. M. Kim, M. Ryu. "Inhibition of Melanogenesis and Anti-UV properties Reynoutria elliptica", Korean Journal of Aesthetics and Cosmetology, Vol.10, No.4 pp.961-968, (2012).
  23. A. Lim, Y. Jung, K. Kim, Y. Kim, J. Kwak, J. Hong, H. Y. Kim, D. Kim, "Skin UVB Photo Aging Effect from Extract of Fermented Reynoutria elliptica", The Korean Society of Food Science and Nutrition, Vol.39, No.3 pp. 369-375, (2010). https://doi.org/10.3746/jkfn.2010.39.3.369
  24. J. Chang, H. Liu, K. Wang, M. Chen, L. Chiang, Y. Hua, C. Lin, "Ethanol Extract of Polygonum cuspidatum Inhibits Hepatitis B virus in a Stable HBV-producing Cell line", Antiviral Research, Vol.66, pp. 29-34, (2005). https://doi.org/10.1016/j.antiviral.2004.12.006
  25. J. Hwang, Y. Park, Y. Kim, J. Kim, C. Lim, "Isolation and Identification of Antifungal Compounds from Reynoutria elliptica", Journal of Agricultural Science, Vol.39, No.4 pp. 583-589, (2012). https://doi.org/10.7744/cnujas.2012.39.4.583
  26. B. R. Kim, J. Ha, S. Lee, J. Park, S. Cho, "Anti-cancer Effects of Ethanol Extract of Reynoutria japonica Houtt. radix in Human Hepatocellular Carcinoma Cells via Inhibition of MAPK and PI3K/Akt Signaling Pathways", Journal of Ethnopharmacology, Vol.245, pp. 112-179, (2019). https://doi.org/10.1016/j.jep.2019.112179
  27. H. Jung, S. Roh, M. Oh, "The Effects of Polygonum Cuspidatum on the Skin Functions", Journal of Korean Medicine Rehabilitation, Vol.19, No.1 pp. 73-89, (2009).
  28. H. Zhang, C. Li, S. Kwok, Q. Zhang, S. Chan, "A Review of the Pharmacological Effects of the Dried Root of Polygonum cuspidatum (Hu Zhang) and Its Constituents", Evidence-Based Complementary and Alternative Medicine, Vol.2013, pp. 13, (2013).
  29. E. Kim, J. Kim, H. K. Kim, Y. Jang "Antimicrobial and Anti-inflammatory Effects of the Ethanol Extract of Polygonum cuspidatum as a Mouthwash Component", Natural Product Communications, Vol.16, No.4 doi:10.1177/1934578X211005255, (2021).
  30. T. Lee, J. Kim, J. So, "Emodin from Polygonum cuspidatum showed Angiogenesis Inhibiting Activity in vitro", The Korean Society for Applied Biological Chemistry, Vol.46, No.1 pp. 50-54, (2003).
  31. C. Hsu, Y. Chan, J. Chang, "Antioxidant Activity of Extract from Polygonum cuspidatum", Biological Research, Vol.40, No.1 pp. 13-21, (2007). https://doi.org/10.4067/S0716-97602007000100002
  32. J. Bak, H. J. Kim, S. Y. Kim, Y. Choi, "Neuroprotective Effect of Caffeic acid Phenethyl Ester in 3-nitropropionic acid-induced Striatal Neurotoxicity", The Korean Journal of Physiology and Pharmacology, Vol.20, No.3 pp. 279-286, (2016). https://doi.org/10.4196/kjpp.2016.20.3.279
  33. S. Kim, J. Choi, Y. Jang, "Emodin Studies on Anti-inflammatory and Skin Improvement Activities", Journal of the Korean Applied Science and Technology, Vol.38, No.6 pp. 1383-1392, (2021).
  34. S. M. Ahn, H. N. Kim, Y. R. Kim, Y. W. Choi, C. M. Kim, H. K. Shin, B. T. Choi, "Emodin from Polygonum multiflorum ameliorates oxidative toxicity in HT22 cells and deficits in photothrombotic ischemia", Journal of Ethnopharmacology, Vol.188, pp. 13-20, (2016). https://doi.org/10.1016/j.jep.2016.04.058
  35. I. Y. Hwang, C. S. Jeong, "A Study on the Antigastritic Effects of Rheum Species Extracts and Their Active Components", Journal of Food Hygiene Safety, Vol.28, No.4 pp. 330-336, (2013). https://doi.org/10.13103/JFHS.2013.28.4.330
  36. K. J. Lee, W. Jang, Y. A. Kim, B. J. Park, H. Kang, "Antioxidant, Inhibitory on NO Production and In-vitro Cell Regeneration Effects of Pink-aloe", Journal of the Society of Cosmetic Scientists of Korea, Vol.46, No.3 pp. 273-282, (2020).