Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Electrochemical Monitoring of NADH Redox with NPQD-modified Electrodes for Cell Viability Assessment

  • JuKyung Lee (Digital Health Care Research Center, Gumi Electronics and Information Technology Research Institute (GERI)) ;
  • Hye Bin Park (Digital Health Care Research Center, Gumi Electronics and Information Technology Research Institute (GERI)) ;
  • Chae Won Seo (Department of Medical IT Convergence, Kumoh National Institute of Technology) ;
  • Chae Won Seo (Department of Medical IT Convergence, Kumoh National Institute of Technology) ;
  • SangHee Kim (Department of Medical IT Convergence, Kumoh National Institute of Technology)
  • Received : 2023.10.31
  • Accepted : 2023.11.10
  • Published : 2023.11.30
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Abstract

There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. Conventional methods depend on optical assays using Water Soluble Tetrazolium-8 (WST-8) or 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, which requires a large volume of samples and special instruments, necessitating shipment of clinical samples to laboratories. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4'-mercapto-N-phenylquinone diamine (4'-NPQD), as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 µM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments.

Keywords

Acknowledgement

This research was supported by Kumoh National Institute of Technology(2022~2023).

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