Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Protective effects of Rosa rugosa THUNB extract on particulate matter-induced skin aging

해당화 추출물의 미세먼지로 유도된 피부 손상 및 노화 억제 효과

  • Yun-Su Lee (Department of Medical Science, Soonchunhyang University) ;
  • Seung Tae Im (Department of Medical Science, Soonchunhyang University) ;
  • Wook chul Kim (Department of Medical Science, Soonchunhyang University) ;
  • Kyeong Min Lee (Department of Pharmaceutical Engineering, Soonchunhyang University) ;
  • Seo Rin Jung (Department of Pharmaceutical Engineering, Soonchunhyang University) ;
  • Hyun-soo Kim (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Seung-Hong Lee (Department of Medical Science, Soonchunhyang University)
  • 이윤수 (순천향대학교 의료과학과) ;
  • 임승태 (순천향대학교 의료과학과) ;
  • 김욱철 (순천향대학교 의료과학과) ;
  • 이경민 (순천향대학교 의약공학과) ;
  • 정서린 (순천향대학교 의약공학과) ;
  • 김현수 (경상국립대학교 해양식품공학과/해양산업연구소) ;
  • 이승홍 (순천향대학교 의료과학과)
  • Received : 2024.07.22
  • Accepted : 2024.09.19
  • Published : 2024.12.31
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Abstract

In recent years, Rosa rugosa has gained recognition as a source of antioxidant phytochemical owing to its abundant phenolic compounds and potent antioxidant activities. However, its protective effects against damage from particulate matter (PM) remain unreported. Therefore, this study focused on the protective effects of R. rugosa ethanol extract (REE) in PM-induced human dermal fibroblasts (HDFs). REE exerted a strong inhibitory effect against DPPH radicals and hydrogen peroxide. It also suppressed the expression of proinflammatory cytokines and matrix metalloproteinases-10 (MMP-10) and promoted collagen synthesis in PM-induced HDFs. Furthermore, the protein expression level of the cell senescence-related marker p53 was reduced in PM-induced HDFs. Furthermore, REE significantly inhibited intracellular reactive oxygen species generation in a PM-treated in vivo zebrafish model, with no observed morphological toxicity. Altogether, these results indicate that REE can be used as a skin antiaging cosmeceutical by suppressing PM-induced collagen degradation and inflammatory responses.

Keywords

Acknowledgement

본 연구는 연구개발특구진흥재단의 '지역혁신 메가프로젝트' 사업으로 수행되었습니다.(과제명:해양바이오 전략소재 메타 플랫폼화 원천기술개발, 과제번호: 2023-DD-UP-0007)

References

  1. Dijkhoff, I. M., Drasler, B., Karakocak, B. B., Petri-Fink, A., Valacchi, G., Eeman, M., & Rothen-Rutishauser, B. 2020. Impact of airborne particulate matter on skin: A systematic review from epidemiology to in vitro studies. Particle and fibre toxicology, 17, 1-28. https://doi.org/10.1186/s12989-019-0331-3
  2. Dai, Y., Wang, Y., Lu, S., Deng, X., Niu, X., Guo, Z., ... & Peng, X. 2021. Autophagy attenuates particulate matter 2.5-induced damage in HaCaT cells. Annals of Translational Medicine, 9(12).
  3. Park, S. Y., Byun, E. J., Lee, J. D., Kim, S., & Kim, H. S. 2018. Air pollution, autophagy, and skin aging: impact of particulate matter (PM10) on human dermal fibroblasts. International journal of molecular sciences, 19(9), 2727.
  4. Kim. K., Lee. C. H., Park. E. Y., Oh. Y. S. 2023. Anti-shinaging effects of Gryllus bimaculatus on ERM-CZ100-exposed human diploid fibroblasts. Journal of Nutrition and Health, 56(6), 615-628. https://doi.org/10.4163/jnh.2023.56.6.615
  5. Wang, J., Huang, J., Wang, L., Chen, C., Yang, D., Jin, M., ... & Song, Y. 2017. Urban particulate matter triggers lung inflammation via the ROS-MAPK-NF-κB signaling pathway. Journal of thoracic disease, 9(11), 4398.
  6. Jung. J.E., Lee. E. B., Kim. S. H., Choi. J. H., Kim. J. S., Kim. J. H. Jeong. U. Y., Choi. H., Lee. Y. S., Lee. S. H. 2022. Antioxidant and Immune-Enhancing Effect of Curcuma longa Extracts. J East Asian Soc Diet Life, 32(3), 137-148. https://doi.org/10.17495/easdl.2022.6.32.3.137
  7. Rui Zhang, Lee. H. J., Yoon. Y. M., Kim. S. M., Kim. H. S., Shun Hua Li, An. S.K. The Melanin Inhibition Anti-aging and Anti-inflammation Effect of Portulaca oleracea Extracts on Cells. KSBB Journal, 24(4), 397-402.
  8. Kim. M. J., Kim. J. S., Kim. K. E., Shin. K. H., Heo. K., Cho. D. H., Park. C. H., Yu C. Y. 2001. Comparison of antioxidative activities from different organs of Rosa rugosa Thunb, 9(1), 40-44.
  9. Cendrowski, A., Ścibisz, I., Mitek, M., Kieliszek, M., & Kolniak-Ostek, J. 2017. Profile of the phenolic compounds of Rosa rugosa petals. Journal of food quality, 2017(1), 7941347.
  10. Blois MS .1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199−1200
  11. Müller HE. 1995. Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl Bakterio Mikrobio Hyg 259: 151−158.
  12. AOAC. 1980. Official methods of analysis. 13th ed, Association of Official Analysis Chemists, Washington, DC, USA. Method 914−915
  13. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  14. Fernando IPS., H. S. Kim., Asanka Sanjeewa KK, J.H. Oh., Y. J. Jeon., W. W. Lee., 2017. Inhibition of inflammatory responses elicited by urban fine dust particles in keratinocytes and macrophages by diphlorethohydroxycarmalol isolated from a brown alga Ishige okamurae. Algae 32(3): 261-273. https://doi.org/10.4490/algae.2017.32.8.14
  15. Shon. M. Y. 2007. Antioxidant Anticancer Activities of Poria cocos and Machilus thundergii Fermented with Mycelial Mushrooms. Food industry and Nutrition, 12(2), 51-57
  16. Mun. H. E., Lee. S. H. 2022. Anti-skin aging activities of ethanol extract from Enhinodorus cordifolius L. in human keratinocytes. Journal of Applied Biological Chemistry, 65(4), 405-412. https://doi.org/10.3839/jabc.2022.052
  17. Lee. S. O., Lee H. J., Yu M. H., Im. H. G., Lee. I. S. 2005. Total Polyphenol Contents and Antioxidant Activities of Methanol Extracts from Vegetables produced in UIIung Island. Food science and technology, 37(2), 233-240
  18. Myong, J. P. 2016. Health effects of particulate matter. The Korean Journal of Medicine, 91(2), 106-113. https://doi.org/10.3904/kjm.2016.91.2.106
  19. Kim, J. Y., Lee, E. Y., Choi, I., Kim, J., & Cho, K. H. 2015. Effects of the particulate matter2. 5 (PM2. 5) on lipoprotein metabolism, uptake and degradation, and embryo toxicity. Molecules and cells, 38(12), 1096-1104. https://doi.org/10.14348/MOLCELLS.2015.0194
  20. Nam, H. Y., Choi, B. H., Lee, J. Y., Lee, S. G., Kim, Y. H., Lee, K. H., ... & Lim, Y. 2004. The role of nitric oxide in the particulate matter (PM2. 5)-induced NFκB activation in lung epithelial cells. Toxicology letters, 148(1-2), 95-102. https://doi.org/10.1016/j.toxlet.2003.12.007
  21. Kim, K. E., Cho, D., & Park, H. J. 2016. Air pollution and skin diseases: Adverse effects of airborne particulate matter on various skin diseases. Life sciences, 152, 126-134. https://doi.org/10.1016/j.lfs.2016.03.039
  22. Park, S. Y., Byun, E. J., Lee, J. D., Kim, S., & Kim, H. S. 2018. Air pollution, autophagy, and skin aging: impact of particulate matter (PM10) on human dermal fibroblasts. International journal of molecular sciences, 19(9), 2727.
  23. Teodoro, J. G., Evans, S. K., & Green, M. R. 2007. Inhibition of tumor angiogenesis by p53: a new role for the guardian of the genome. Journal of molecular medicine, 85, 1175-1186. https://doi.org/10.1007/s00109-007-0221-2
  24. Itahana, K., Dimri, G., & Campisi, J. 2001. Regulation of cellular senescence by p53. European journal of biochemistry, 268(10), 2784-2791. https://doi.org/10.1046/j.1432-1327.2001.02228.x
  25. Atadja, P., Wong, H., Garkavtsev, I., Veillette, C., & Riabowol, K. 1995. Increased activity of p53 in senescing fibroblasts. Proceedings of the National Academy of Sciences, 92(18), 8348-8352. https://doi.org/10.1073/pnas.92.18.8348
  26. McGrath, P., & Li, C. Q. 2008. Zebrafish: a predictive model for assessing drug-induced toxicity. Drug discovery today, 13(9-10), 394-401. https://doi.org/10.1016/j.drudis.2008.03.002
  27. Lardelli, M. 2008. Using zebrafish in human disease research: some advantages, disadvantages and ethical considerations. In Proceedings of 2008 ANZCCART Conference, Auckland, New Zealand pp. 23-28.
  28. Zhou, Y., Chen, X., Teng, M., Zhang, J., & Wang, C. 2019. Toxicity effects of captan on different life stages of zebrafish (Danio rerio). Environmental toxicology and pharmacology, 69, 80-85. https://doi.org/10.1016/j.etap.2019.04.003
  29. Piao, M. J., Ahn, M. J., Kang, K. A., Ryu, Y. S., Hyun, Y. J., Shilnikova, K., ... & Hyun, J. W. 2018. Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis. Archives of toxicology, 92, 2077-2091. https://doi.org/10.1007/s00204-018-2197-9