Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts

  • Dohyun Kim (Department of Animal Biotechnology, Chonbuk National University) ;
  • HakKyo Lee (Department of Animal Biotechnology, Chonbuk National University) ;
  • Dae-Jin Kwon (Department of Animal Biotechnology, Chonbuk National University)
  • Received : 2024.11.26
  • Accepted : 2024.12.22
  • Published : 2024.12.31
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Abstract

Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts. Methods: In vitro fertilized (IVF) eggs were cultured in IVC medium (control) with the addition of mTeSR1 supplement at concentrations of 1%, 2%, and 5%, respectively. The development rates of fertilized eggs and gene expression patterns of blastocysts were assessed on day 9 of culture. For outgrowth culture, blastocysts were cultured on a mouse embryonic fibroblast feeder cells (MEFs) for 7 days. Results: In vitro development of bovine preimplantation embryos in the 2% mTeSR1 group was significantly higher than in the control (p < 0.05). The apoptotic index in the 2% mTeSR1 group was significantly lower compared to the control (p < 0.05). RT-qPCR indicated that SRY-Box Transcription Factor 2 (Sox2) gene expression in the 5% mTeSR1 group was significantly higher than in the control (p < 0.05). The 5% mTeSR1 group also showed significantly higher BCL2 associated X (Bax) expression compared to the control and other mTeSR1 groups. On day 9 pi, blastocysts from the control and 2% mTeSR1 groups were cultured for 7 days. The 2% mTeSR1 group showed higher efficiency in forming dome-shaped colonies with stronger SOX2 expression compared to the control. Conclusions: The mTeSR1 supplement supports preimplantation embryo development and prevents apoptosis in blastocysts, leading to the efficient formation of dome-shaped inner cell mass (ICM) colonies.

Keywords

Acknowledgement

This work was carried out with the support of the "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01620003)" Rural Development Administration, Republic of Korea, and supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2020R1I1A1A01070671).

References

  1. Barrera AD, García EV, Miceli DC. 2018. Effect of exogenous transforming growth factor β1 (TGF-β1) on early bovine embryo development. Zygote 26:232-241. https://doi.org/10.1017/S096719941800014X
  2. Bhang SH, Lee S, Shin JY, Lee TJ, Jang HK, Kim BS. 2014. Efficacious and clinically relevant conditioned medium of human adipose-derived stem cells for therapeutic angiogenesis. Mol. Ther. 22:862-872. https://doi.org/10.1038/mt.2013.301
  3. Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, Ross PJ. 2018. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc. Natl. Acad. Sci. U. S. A. 115:2090-2095. https://doi.org/10.1073/pnas.1716161115
  4. Botigelli RC, Guiltinan C, Arcanjo RB, Denicol AC. 2023. In vitro gametogenesis from embryonic stem cells in livestock species: recent advances, opportunities, and challenges to overcome. J. Anim. Sci. 101:skad137. https://doi.org/10.1093/jas/skad137
  5. Goissis MD and Cibelli JB. 2014. Functional characterization of CDX2 during bovine preimplantation development in vitro. Mol. Reprod. Dev. 81:962-970. https://doi.org/10.1002/mrd.22415
  6. Hajian M, Hosseini SM, Ostadhosseini S, Nasr-Esfahani MH. 2016. Targeting the transforming growth factor-β signaling during pre-implantation development in embryos of cattle, sheep and goats. Growth Factors 34:141-148. https://doi.org/10.1080/08977194.2016.1206089
  7. Ikonomovic S, Kharlamov E, Manev H, Ikonomovic MD, Grayson DR. 1997. GABA and NMDA in the prevention of apoptotic-like cell death in vitro. Neurochem. Int. 31:283-290. https://doi.org/10.1016/S0197-0186(96)00159-3
  8. Isaac E, Berg DK, Pfeffer PL. 2024. Using extended growth of cattle embryos in culture to gain insights into bovine developmental events on embryonic days 8 to 10. Theriogenology 214:10-20. https://doi.org/10.1016/j.theriogenology.2023.10.004
  9. Khan DR, Dubé D, Gall L, Peynot N, Ruffini S, Laffont L, Le Bourhis D, Degrelle S, Jouneau A, Duranthon V. 2012. Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo. PLoS One 7:e34110. https://doi.org/10.1371/journal.pone.0034110
  10. Kwon DJ, Hwang IS, Kwak TU, Oh KB, Ock SA, Chung HJ, Im GS, Hwang S. 2015. Effect of stem cell-derived conditioned medium on the in vitro maturation and embryonic development of parthenogenetic embryos in pigs. Reprod. Dev. Biol. 39:89-95. https://doi.org/10.12749/RDB.2015.39.3.89
  11. Kwong WY, Adamiak SJ, Gwynn A, Singh R, Sinclair KD. 2010. Endogenous folates and single-carbon metabolism in the ovarian follicle, oocyte and pre-implantation embryo. Reproduction 139:705-715. https://doi.org/10.1530/REP-09-0517
  12. Landecker HL and Clark AT. 2023. Human embryo models made from pluripotent stem cells are not synthetic; they aren't embryos, either. Cell Stem Cell 30:1290-1293. https://doi.org/10.1016/j.stem.2023.09.006
  13. Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA. 2006. Feeder-independent culture of human embryonic stem cells. Nat. Methods. 3:637-646. https://doi.org/10.1038/nmeth902
  14. Luo L, Shi Y, Wang H, Wang Z, Dang Y, Li S, Wang S, Zhang K. 2022. Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency. PLoS Genet. 18:e1010307. https://doi.org/10.1371/journal.pgen.1010307
  15. Martal JL, Chêne NM, Huynh LP, L'Haridon RM, Reinaud PB, Guillomot MW, Charlier MA, Charpigny SY. 1998. IFN-tau: a novel subtype I IFN1. Structural characteristics, nonubiquitous expression, structure-function relationships, a pregnancy hormonal embryonic signal and cross-species therapeutic potentialities. Biochimie 80:755-777. https://doi.org/10.1016/S0300-9084(99)80029-7
  16. Neira JA, Tainturier D, Peña MA, Martal J. 2010. Effect of the association of IGF-I, IGF-II, bFGF, TGF-beta1, GM-CSF, and LIF on the development of bovine embryos produced in vitro. Theriogenology 73:595-604. https://doi.org/10.1016/j.theriogenology.2009.10.015
  17. Ramos-Ibeas P, Pérez-Gómez A, González-Brusi L, Quiroga AC, Bermejo-Álvarez P. 2023. Pre-hatching exposure to N2B27 medium improves post-hatching development of bovine embryos in vitro. Theriogenology 205:73-78. https://doi.org/10.1016/j.theriogenology.2023.04.018
  18. Soto DA and Ross PJ. 2016. Pluripotent stem cells and livestock genetic engineering. Transgenic Res. 25:289-306. https://doi.org/10.1007/s11248-016-9929-5
  19. Tian N, Liang H, Luo W, Wang X, Cao K, Zhang Q, Tan Y, Tan D. 2020. GABA consumption during early pregnancy impairs endometrial receptivity and embryo development in mice. J. Biochem. Mol. Toxicol. 34:e22473. https://doi.org/10.1002/jbt.22473
  20. Treleaven T, Hardy MLM, Guttman-Jones M, Morris MB, Day ML. 2021. In vitro fertilisation of mouse oocytes in L-proline and L-pipecolic acid improves subsequent development. Cells 10:1352. https://doi.org/10.3390/cells10061352
  21. Uhm SJ. 2023. Effect of supplement of SCM in culture medium for in vitro development of bovine in vitro fertilized oocytes. J. Anim. Reprod. Biotechnol. 38:143-150. https://doi.org/10.12750/JARB.38.3.143
  22. Vallier L, Reynolds D, Pedersen RA. 2004. Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway. Dev. Biol. 275:403-421. https://doi.org/10.1016/j.ydbio.2004.08.031
  23. Yang QE, Fields SD, Zhang K, Ozawa M, Johnson SE, Ealy AD. 2010. FGF2 promotes primitive endoderm development in bovine blastocysts. Biol. Reprod. 83(Suppl_1):181. 24. https://doi.org/10.1093/biolreprod/83.s1.181
  24. Zhao L, Gao X, Zheng Y, Wang Z, Zhao G, Ren J, Zhang J, Wu J, Wu B, Chen Y, Sun W, Li Y, Su J, Ding Y, Gao Y, Liu M, Bai X, Sun L, Cao G, Tang F, Bao S, Liu P, Li X. 2021. Establishment of bovine expanded potential stem cells. Proc. Natl. Acad. Sci. U. S. A. 118:e2018505118. https://doi.org/10.1073/pnas.2018505118
  25. Zhu M and Zernicka-Goetz M. 2020. Principles of self-organization of the mammalian embryo. Cell 183:1467-1478. https://doi.org/10.1016/j.cell.2020.11.003