The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Genetic Variation of Monilinia fructicola Population in Korea

  • Su In Lee (Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Hwa-Jung Lee (Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Youn-Sig Kwak (Division of Applied Life Science (BK21Plus), Gyeongsang National University)
  • 투고 : 2024.01.20
  • 심사 : 2024.03.08
  • 발행 : 2024.04.01
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초록

Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicola isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.

키워드

과제정보

This research was supported by the Rural Development Administration of Korea (RS-2022-RD010350).

참고문헌

  1. Basu, A., Ghosh, M., Meyer, R., Powell, W., Basak, S. L. and Sen, S. K. 2004. Analysis of genetic diversity in cultivated jute determined by means of SSR markers and AFLP profiling. Crop Sci. 44:678-685.
  2. Choi, I.-Y., Kim, J., Seo, K.-W., Oh, H.-T., Cho, J.-H., Kim, J.- H. and Song, Y.-J. 2016. Occurrence of brown rot on apricot caused by Monilinia fructicola in Korea. Res. Plant Dis. 22:122-126 (in Korean).
  3. Cote, M.-J., Tardif, M.-C. and Meldrum, A. J. 2004. Identification of Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma on inoculated and naturally infected fruit using multiplex PCR. Plant Dis. 88:1219-1225.
  4. De Miccolis Angelini, R. M., Abate, D., Rotolo, C., Gerin, D., Pollastro, S. and Faretra, F. 2018. De novo assembly and comparative transcriptome analysis of Monilinia fructicola, Monilinia laxa and Monilinia fructigena, the causal agents of brown rot on stone fruits. BMC Genomics 19:436.
  5. Fischer, J. M. M., Savi, D. C., Aluizio, R., May De Mio, L. L. and Glienke, C. 2017. Characterization of Monilinia species associated with brown rot in stone fruit in Brazil. Plant Pathol. 66:423-436.
  6. Fulton, C. E. and Brown, A. E. 1997. Use of SSU rDNA group-l intron to distinguish Monilinia fructicola from M. laxa and M. fructigena. FEMS Microbiol. Lett. 157:307-312.
  7. Fulton, C. E., van Leeuwen, G. C. M. and Brown, A. E. 1999. Genetic variation among and within Monilinia species causing brown rot of stone and pome fruits. Eur. J. Plant Pathol. 105:495-500.
  8. Gell, I., Cubero, J. and Melgarejo, P. 2007. Two different PCR approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees. J. Appl. Microbiol. 103:2629-2637.
  9. Gril, T., Celar, F., Javornik, B. and Jakse, J. 2010. Fluorescent AFLP fingerprinting of Monilinia fructicola. J. Plant Dis. Prot. 117:168-172.
  10. Gril, T., Celar, F., Munda, A., Javornik, B. and Jakse, J. 2008. AFLP analysis of intraspecific variation between Monilinia laxa isolates from different hosts. Plant Dis. 92:1616-1624.
  11. Hily, J.-M., Singer, S. D., Vilani, S. M. and Cox, K. D. 2011. Characterization of the cytochrome b (cyt b) gene from Monilinia species causing brown rot of stone and pome fruit and its significance in the development of QoI resistance. Pest Manag. Sci. 67:385-396.
  12. Hrustic. J., Mihajlovic, M., Grahovac, M., Delibasic, G. and Tanovic, B. 2018. Fungicide sensitivity, growth rate, aggressiveness and frost hardiness of Monilinia fructicola and Monilinia laxa isolates. Eur. J. Plant Pathol. 151:389-400.
  13. Hu, M.-J., Cox, K. D., Schnabel, G. and Luo, C.-X. 2011. Monilinia species causing brown rot of peach in China. PLoS ONE 6:e24990.
  14. Jones, A. L. and Ehret, G. R. 1976. Isolation and characterization of benomyl-tolerant strains of Monilinia fructicola. Plant Dis. Rep. 60:765-769.
  15. Kim, C. H. 2000. Review of fungicide resistance problems in Korea. Korean J. Pestic. Sci. 4:1-10 (in Korean).
  16. Kumar, P., Gupta, V. K., Misra, A. K., Modi, D. R. and Pandey, B. K. 2009. Potential of molecular markers in plant biotechnology. Plant Omics J. 2:141-162.
  17. Lane, C. R. 2002. A synoptic key for differentiation of Monilinia fructicola, M. fructigena and M. laxa, based on examination of cultural characters. EPPO Bull. 32:489-493.
  18. Lee, S. I. and Kwak, Y.-S. 2023. Evaluation of fungicides sensitivities of peach brown rot pathogen, Monilinia fructicola. Korean J. Pestic. Sci. 27:154-162 (in Korean).
  19. Lesniak, K. E., Peng, J., Proffer, T. J., Outwater, C. A., Eldred, L. I., Rothwell, N. L. and Sundin, G. W. 2021. Survey and genetic analysis of demethylation inhibitor fungicide resistance in Monilinia fructicola from Michigan orchards. Plant Dis. 105:958-964.
  20. Lim, T. H., Chang, T. H. and Cha, B. 1998. Incidence of benzimidazole- and dicarboximide resistant isolates of Monilinia fructicola from overwintering mummies and peduncles on peach tree. Korean J. Plant Pathol. 14:367-370 (in Korean).
  21. Luo, C.-X. and Schnabel, G. 2008. The cytochrome P450 lanosterol 14α-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgi. Appl. Environ. Microbiol. 74:359-366.
  22. Majer, D., Mithen, R., Lewis, B. G., Vos, P. and Oliver, R. P. 1996. The use of AFLP fingerprinting for the detection of genetic variation in fungi. Mycol. Res. 100:1107-1111.
  23. Martini, C. and Mari, M. 2014. Monilinia fructicola, Monilinia laxa (Monilinia rot, brown rot). In: Postharvest decay: control strategies, ed. by S. Bautista-Banos, pp. 233-265. Academic Press, Waltham, MA, USA.
  24. Oh, H.-T., Choi, I.-Y., Kim, J., Na, Y.-E., Lee, W.-H., Lee, K.-J. and Shin, H.-D. 2017. Characteristics of brown rot caused by Monilinia fructicola on stone fruit in Korea. Res. Plant Dis. 23:322-333 (in Korean).
  25. Poniatowska, A., Michalecka, M. and Pulawska, J. 2021. Phylogenetic relationships and genetic diversity of Monilinia spp. isolated in Poland based on housekeeping- and pathogenicity-related gene sequence analysis. Plant Pathol. 70:1640-1650.
  26. Purwantara, A., Barrins, J. M., Cozijnsen, A. J. and Howle, B. J. 2000. Genetic diversity of isolates of the Leptosphaeria maculans species complex from Australia, Europe and North America using amplified fragment length polymorphism analysis. Mycol. Res. 104:772-781.
  27. Rural Development Administration. 2007. Monography of stone fruit cultivars. Rural Development Administration, Suwon, Korea. 252 pp. (in Korean).
  28. Sammour, R. H., Badr, S., Mustafa, A. E.-Z. and El-Esawi, M. 2013. Genetic variation within and among some Lactuca spp. based on karyotype analysis. Appl. Cell Biol. 2:136-143.
  29. Schnabel, G., Bryson, P. K., Bridges, W. C. and Brannen, P. M. 2004. Reduced sensitivity in Monilinia fructicola to propiconazole in Georgia and implications for disease management. Plant Dis. 88:1000-1004.
  30. Schnieder, F., Koch, G., Jung, C. and Verreet, J.-A. 2001. Genotypic diversity of the wheat leaf blotch pathogen Mycosphaerella graminicola (anamorph) Septoria tritici in Germany. Eur. J. Plant Pathol. 107:285-290.
  31. Shim, M. Y., Jeon, Y. J. and Kim, S, H, 2007. Characterization of a brown rot fungus isolated from dwarf flowering almond in Korea. Mycobiology 35:30-35.
  32. Sztejnberg, A. and Jones, A, L. 1978. Tolerance of the brown rot fungus Monilinia fructiocola to iprodione, vinclozolin and procymidone fungicides. Phytopathol. News 12:187-188.
  33. Usman, H. M., Tan, Q., Karim, M. M., Adnan, M., Yin, W.-X., Zhu, F.-X. and Luo, C.-X. 2021. Sensitivity of Colletotrichum fructicola and Colletotrichum siamense of peach in China to multiple classes of fungicides and characterization of pyraclostrobin resistant isolates. Plant Dis. 105:3459-3465.
  34. van Leeuwen, G. C. M., Baayen, R. P. and Jeger, M, J. 2002. Distinction of the Asiatic brown rot fungus Monilia polystroma sp. nov. from M. fructigena. Mycol. Res. 106:444-451.
  35. Vos, P., Hogers, R., Bleeker, M. Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414.
  36. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
  37. Zhu, X. Q., Chen, X. Y., Luo, Y. and Guo, L. Y. 2005. First report of Monilinia fructicola on peach and nectarine in China. Plant Pathol. 54:575.