Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant, Anti-inflammation, and Anti-aging Effects of Ethyl Acetate Fraction from Cynanchum paniculatum Extracts

산해박 분획물의 항산화, 항염증 및 항노화 활성 효과 연구

  • Jae Yong Seo (CIR Center, COSMECCA KOREA) ;
  • Go Eun Kim (CIR Center, COSMECCA KOREA) ;
  • Ji Soo Ryu (CIR Center, COSMECCA KOREA) ;
  • A Reum Jang (CIR Center, COSMECCA KOREA) ;
  • Su An Sim (Bio Convergence R&D Center, CoSeedBioPharm Corporation) ;
  • Jung No Lee (Bio Convergence R&D Center, CoSeedBioPharm Corporation) ;
  • Seunghee Bae (Department of Cosmetics Engineering, Konkuk University)
  • 서재용 ((주)코스메카코리아) ;
  • 김고은 ((주)코스메카코리아) ;
  • 유지수 ((주)코스메카코리아) ;
  • 장아름 ((주)코스메카코리아) ;
  • 심수안 ((주)코씨드바이오팜 바이오융합연구소) ;
  • 이정노 ((주)코씨드바이오팜 바이오융합연구소) ;
  • 배승희 (건국대학교 화장품공학과)
  • Received : 2024.03.14
  • Accepted : 2024.03.30
  • Published : 2024.03.30
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Abstract

In this study, we prepared fractions from Cynanchum paniculatum (C. paniculatum) with ethyl acetate and identified the structure and content of paeonol, an indicator substance. Fractions were obtained by sequential solvent fractions using n-hexane, methylene chloride, ethyl acetate, n-butanol, and distilled water, and showed the highest paeonol content in ethyl acetate (EtOAc). The structure of the ethyl acetate fraction (EA) was confirmed by nuclear magnetic resonance (NMR) and the content was determined by high performance liquid chromatography (HPLC) analysis. The antioxidant activity of EA was tested through its ability to scavenge 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and its ability to inhibit nitric oxide (NO) production in RAW 264.7 cells induced by lipopolysaccharide (LPS) was tested for its ability to inhibit NO production in a concentration-dependent manner. Furthermore, MMP-1 production induced by ultraviolet B (UVB) was decreased in a concentration-dependent manner by the EA, and the ability to produce procollagen type-I was increased in a concentration-dependent manner. In addition, as a result of conducting clinical trials using cosmetic formulations containing EA, the effect of improving wrinkles around the eyes was confirmed. Thus, it is expected that Cynanchum paniculatum can be used as a natural functional cosmetic material with antioxidant and anti-aging effects.

본 연구에서는 산해박(Cynanchum paniculatum)을 이용하여 에틸아세테이트로 분획물을 제조하고, 지표물질 페오놀(paeonol)의 구조와 함량을 확인하였다. 산해박은 n-hexane, methylene chloride, ethyl acetate, n-butanol, distilled water를 사용해서 순차적 용매 분획을 시행하여 분획물을 획득하였고, 에틸아세테이트(EtOAc)에서 가장 높은 paeonol 함량을 보였다. 산해박 에틸아세테이트 분획물(EA)의 구조는 nuclear magnetic resonance (NMR)을 통해서 확인하였고, 함량은 high performance liquid chromatography (HPLC) 분석을 통해 확인하였다. 산해박 에틸아세테이트 분획물(EA)의 항산화 활성 효과를 검증하기 위해 2,2'-azino-bis(3-ethylbenzothiazoline6-sulphonic acid) (ABTS) 소거능을 통해서 확인하였으며, 염증 완화 효과를 검증하기 위해 lipopolysaccharide (LPS)로 염증반응을 유도시킨 RAW 264.7 세포에서 nitric oxide (NO) 생성 억제능을 확인한 결과, 농도 의존적으로 NO 생성을 저해함을 확인하였다. 추가적으로, ultraviolet B (UVB)에 의해 유도된 MMP-1 생성량은 산해박 에틸아세테이트 분획물(EA)의 농도 의존적으로 감소하였고, 프로콜라겐(procollagen type-I) 생성 능력은 농도 의존적으로 증가하는 것을 확인하였다. 또한 산해박 에틸아세테이트 분획물(EA)을 함유한 화장품 제형을 이용하여 임상시험을 실시한 결과, 눈가 주름 개선 효과를 확인하였다. 이를 통하여 산해박은 항산화 및 항노화 효과를 가지는 천연 기능성 화장품 소재로 사용이 가능할 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 산업통상자원부에서 지원하는 산업기술혁신사업(10077377)의 지원을 받아서 수행된 연구 결과 입니다.

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