Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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A novel p.127Val>Ile single nucleotide polymorphism in the MTNR1A gene and its relation to litter size in Thin-tailed Indonesian ewes

  • Mutasem Abuzahra (Faculty of Veterinary Medicine, Universitas Airlangga) ;
  • Mohammed Baqur S. Al-Shuhaib (Department of Animal Production, College of Agriculture, Al-Qasim Green University) ;
  • Dwi Wijayanti (College of Animal Science and Technology, Northwest A&F University) ;
  • Mustofa Helmi Effendi (Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga) ;
  • Imam Mustofa (Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University) ;
  • Ikechukwu Benjamin Moses (Department of Applied Microbiology, Faculty of Science, Ebonyi State University)
  • Received : 2024.03.29
  • Accepted : 2024.06.07
  • Published : 2025.02.01
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Abstract

Objective: The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait. Methods: Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene. Results: The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin. Conclusion: In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection.

Keywords

Acknowledgement

This study was supported in part by the Skema Penelitian Unggulan Airlangga (PUA) Universitas Airlangga in year 2023 ( 1710/UN3.LPPM/ PT.01.03/2023).

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