Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Microbial Interactions in the Green Peach Aphid Myzus persicae Sulzer (Hemiptera: Aphididae) Insights from Microbiome Analysis

복숭아혹진딧물의 미생물 상호작용: 미생물 군집분석을 통한 통찰

  • Byung-Hyuk Kim (National Institute of Horticultural and Herbal Science, RDA) ;
  • Jong-Ok Jang (Division of Horticulture & Medicinal Plant, Andong National University) ;
  • Jung-Eun Kim (National Institute of Horticultural and Herbal Science, RDA) ;
  • Chun Hwan Kim (National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyun-Hee Han (National Institute of Horticultural and Herbal Science, RDA) ;
  • Jeong Joon Ahn (National Institute of Horticultural and Herbal Science, RDA)
  • 김병혁 (제주시 농촌진흥청, 국립원예특작과학원, 온난화대응농업연구소) ;
  • 장종옥 (국립안동대학교 원예 및 약용식물학과) ;
  • 김정은 (제주시 농촌진흥청, 국립원예특작과학원, 온난화대응농업연구소) ;
  • 김천환 (제주시 농촌진흥청, 국립원예특작과학원, 온난화대응농업연구소) ;
  • 한현희 (제주시 농촌진흥청, 국립원예특작과학원, 온난화대응농업연구소) ;
  • 안정준 (제주시 농촌진흥청, 국립원예특작과학원, 온난화대응농업연구소)
  • Received : 2024.11.27
  • Accepted : 2025.02.10
  • Published : 2025.03.01
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Abstract

The green peach aphid (Myzus persicae Sulzer, Hemiptera: Aphididae) is a significant agricultural pest, inflicting economic loss on vegetable crops, fruit trees, and ornamental plants. To elucidate the interactions between M. persicae and its endosymbiotic microbial communities, the microbiota of aphids maintained in isolated rearing conditions for approximately two years were analyzed using 16S rRNA gene sequencing. The dominant microbial taxa identified included γ-proteobacteria, such as Buchnera sp., Pseudomonas sp., and Propionibacterium sp. Quantitative PCR revealed that the bacterial community size was approximately 4,300 times greater than the fungal community size. Among these, Buchnera sp., a γ-proteobacterium, exhibited a highly intimate symbiotic relationship with the host aphid. These findings contribute foundational knowledge toward understanding the interactinos between M. persicae and its associated microbial communities, with implications for the management of this pest species.

복숭아혹진딧물(Myzus persicae Sulzer, Hemiptera: Aphididae)은 채소작물, 과수작물과 화훼작물에 발생하여 농업과 원예작물에 경제적 피해를 주는 주요 해충이다. 복숭아혹진딧물과 기주내부 미생물간의 상호작용을 이해하기 위해, 격리된 사육시설에서 약 2년간 누대사육된 복숭아 혹진딧물 내의 미생물 군집구조는 16S rRNA gene을 이용하여 분석하였다. 복숭아혹진딧물에 우점하는 미생물은 γ-proteobacteria에 속하는 Buchnera sp., Pseudomonas sp., Propionibacterium sp. 등이 주요 우점하는 것을 확인되었다. 미생물 군집크기분석은 정량 PCR을 통해 분석하였으며, 박테리아 군집의 크기가 곰팡이 군집보다 약 4,300배 큰 것을 확인하였다. 이러한 결과로부터 복숭아혹진딧물 내부에는 γ-proteobacteria에 속하는 Buchnera sp.가 기주와 매우 밀접한 상호관계가가 이루어진다고 판단되어진다. 본 연구의 결과는 복숭아혹진딧물과 기주내부 미생물간의 상호작용을 이해하는데 기초자료로 제공될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 시험연구과제인 '기온 및 이산화탄소 변화에 따른 복숭아혹진딧물과 기생벌 상호작용 영향평가(과제번호: PJ01606001)'로 수행되었으며, 2024년 농촌진흥청 국립원예특작과학원 전문연구원 과정 지원사업에 의해 이루어진 것입니다.

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